Resveratrol protects rats from Aβ-induced neurotoxicity by the reduction of iNOS expression and lipid peroxidation

Tai Chun Huang, Kwok-Tung Lu, Yu Yuan Peter Wo, Yao Ju Wu, Yi Ling Yang

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Abstract

Alzheimer disease (AD) is an age-dependent neurodegenerative disease characterized by the formation of β-amyloid (Aβ)-containing senile plaque. The disease could be induced by the administration of Aβ peptide, which was also known to upregulate inducible nitric oxide synthase (iNOS) and stimulate neuronal apoptosis. The present study is aimed to elucidate the cellular effect of resveratrol, a natural phytoestrogen with neuroprotective activities, on Aβ-induced hippocampal neuron loss and memory impairment. On adult Sprague-Dawley rats, we found the injection of Aβ could result in a significant impairment in spatial memory, a marked increase in the cellular level of iNOS and lipid peroxidation, and an apparent decrease in the expression of heme oxygenase-1 (HO-1). By combining the treatment with Aβ, resveratrol was able to confer a significant improvement in spatial memory, and protect animals from Aβ-induced neurotoxicity. These neurological protection effects of resveratrol were associated with a reduction in the cellular levels of iNOS and lipid peroxidation and an increase in the production of HO-1. Moreover, the similar neurological and cellular response were also observed when Aβ treatment was combined with the administration of a NOS inhibitor, N(G)-nitro-L-arginine methyl ester hydrochloride (L-NAME). These findings strongly implicate that iNOS is involved in the Aβ-induced lipid peroxidation and HO-1 downregulation, and resveratrol protects animals from Aβ-induced neurotoxicity by suppressing iNOS production.

Original languageEnglish
Article numbere29102
JournalPLoS ONE
Volume6
Issue number12
DOIs
Publication statusPublished - 2011 Dec 29

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neurotoxicity
resveratrol
heme oxygenase (biliverdin-producing)
Nitric Oxide Synthase Type II
Lipid Peroxidation
Rats
lipid peroxidation
Heme Oxygenase-1
Lipids
rats
NG-Nitroarginine Methyl Ester
Data storage equipment
Animals
Neurodegenerative diseases
Phytoestrogens
neuroprotective effect
plant estrogens
Amyloid Plaques
Memory Disorders
neurodegenerative diseases

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Resveratrol protects rats from Aβ-induced neurotoxicity by the reduction of iNOS expression and lipid peroxidation. / Huang, Tai Chun; Lu, Kwok-Tung; Wo, Yu Yuan Peter; Wu, Yao Ju; Yang, Yi Ling.

In: PLoS ONE, Vol. 6, No. 12, e29102, 29.12.2011.

Research output: Contribution to journalArticle

Huang, Tai Chun ; Lu, Kwok-Tung ; Wo, Yu Yuan Peter ; Wu, Yao Ju ; Yang, Yi Ling. / Resveratrol protects rats from Aβ-induced neurotoxicity by the reduction of iNOS expression and lipid peroxidation. In: PLoS ONE. 2011 ; Vol. 6, No. 12.
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