Relationship between antibacterial activity of chitosan and surface characteristics of cell wall

Ying Chien Chung, Ya Ping Su, Chiing Chang Chen, Guang Jia, Huey Lan Wang, J. C.Gaston Wu, Jaung Geng Lin

Research output: Contribution to journalArticle

360 Citations (Scopus)

Abstract

AIM: Five representative waterborne pathogens were used to illustrate the relationship between chitosan's antibacterial activity and the surface characteristics of the bacterial cell wall. METHODS: Chitosan was prepared with averaged 75 % or 95 % deacetylated degree to examine its antibacterial activity against waterborne pathogens. Fresh microbial inoculants for the antibacterial assessment were prepared on nutrient agar at 37 °C for 24 h. The evaluation items of antibacterial mechanism included hydrophilicity and negative charge analysis of cell surface, and adsorptive characteristics of chitosan to bacterial cell. All the experiments were applied in triplicate tests at least. RESULTS: Although cell wall hydrophilicity was similar among Gram-negative bacteria, the distribution of negative charge on their cell surfaces was quite different. More negatively charged cell surfaces had a greater interaction with chitosan, a phenomenon further confirmed by transmission electron micrography (TEM). CONCLUSION: Results showed the hydrophilicity in Gram-negative bacteria was much higher than in Gram-positive ones. The correlation coefficient 0.988 between the amount of absorbed chitosan and its inhibition efficiency indicated a close relationship.

Original languageEnglish
Pages (from-to)932-936
Number of pages5
JournalActa Pharmacologica Sinica
Volume25
Issue number7
Publication statusPublished - 2004 Jul 1

Fingerprint

Chitosan
Cell Wall
Cells
Hydrophilicity
Hydrophobic and Hydrophilic Interactions
Pathogens
Gram-Negative Bacteria
Bacteria
Agricultural Inoculants
Nutrients
Agar
Electrons
Food
Experiments

Keywords

  • Anti-bacterial agents
  • Cell wall
  • Chitosan

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Chung, Y. C., Su, Y. P., Chen, C. C., Jia, G., Wang, H. L., Wu, J. C. G., & Lin, J. G. (2004). Relationship between antibacterial activity of chitosan and surface characteristics of cell wall. Acta Pharmacologica Sinica, 25(7), 932-936.

Relationship between antibacterial activity of chitosan and surface characteristics of cell wall. / Chung, Ying Chien; Su, Ya Ping; Chen, Chiing Chang; Jia, Guang; Wang, Huey Lan; Wu, J. C.Gaston; Lin, Jaung Geng.

In: Acta Pharmacologica Sinica, Vol. 25, No. 7, 01.07.2004, p. 932-936.

Research output: Contribution to journalArticle

Chung, YC, Su, YP, Chen, CC, Jia, G, Wang, HL, Wu, JCG & Lin, JG 2004, 'Relationship between antibacterial activity of chitosan and surface characteristics of cell wall', Acta Pharmacologica Sinica, vol. 25, no. 7, pp. 932-936.
Chung, Ying Chien ; Su, Ya Ping ; Chen, Chiing Chang ; Jia, Guang ; Wang, Huey Lan ; Wu, J. C.Gaston ; Lin, Jaung Geng. / Relationship between antibacterial activity of chitosan and surface characteristics of cell wall. In: Acta Pharmacologica Sinica. 2004 ; Vol. 25, No. 7. pp. 932-936.
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