Regulation of glycogen metabolism in gills and liver of the euryhaline tilapia (Oreochromis mossambicus) during acclimation to seawater

Joshua Chia Hsi Chang, Su Mei Wu, Yung-Che Tseng, Yi Chun Lee, Otto Baba, Pung Pung Hwang

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Glucose, which plays a central role in providing energy for metabolism, is primarily stored as glycogen. The synthesis and degradation of glycogen are mainly initialized by glycogen synthase (GS) and glycogen Phosphorylase (GP), respectively. The present study aimed to examine the glycogen metabolism in fish liver and gills during acute exposure to seawater. In tilapia (Oreochromis mossambicus) gill, GP, GS and glycogen were immunocytochemically colocalized in a specific group of glycogen-rich (GR) cells, which are adjacent to the gill's main ionocytes, mitochondrion-rich (MR) cells. Na + /K + -ATPase activity in the gills, protein expression and/or activity of GP and GS and the glycogen content of the gills and liver were examined in tilapia after their acute transfer from freshwater (FW) to 25‰ seawater (SW). Gill Na + /K + -ATPase activity rapidly increased immediately after SW transfer. Glycogen content in both the gills and liver were significantly depleted after SW transfer, but the depletion occurred earlier in gills than in the liver. Gill GP activity and protein expression were upregulated 1-3 h posttransfer and eventually recovered to the normal level as determined in the control group. At the same time, GS protein expression was downregulated. Similar changes in liver GP and GS protein expression were also observed but they occurred later at 6-12 h post-transfer. In conclusion, GR cells are initially stimulated to provide prompt energy for neighboring MR cells that trigger ion-secretion mechanisms. Several hours later, the liver begins to degrade its glycogen stores for the subsequent energy supply.

Original languageEnglish
Pages (from-to)3494-3504
Number of pages11
JournalJournal of Experimental Biology
Volume210
Issue number19
DOIs
Publication statusPublished - 2007 Oct 1

Fingerprint

Tilapia (Cichlidae)
Tilapia
Oreochromis mossambicus
Acclimatization
Seawater
Glycogen
acclimation
glycogen
gills
seawater
metabolism
Glycogen Phosphorylase
Glycogen Synthase
glycogen (starch) synthase
liver
protein
Liver
mitochondrion
phosphorylase
secretion

Keywords

  • Glycogen phosphorylase
  • Glycogen synthase
  • Mitochondrion-rich cells
  • Na /K -ATPase
  • Osmoregulation
  • Salinity

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

Cite this

Regulation of glycogen metabolism in gills and liver of the euryhaline tilapia (Oreochromis mossambicus) during acclimation to seawater. / Chang, Joshua Chia Hsi; Wu, Su Mei; Tseng, Yung-Che; Lee, Yi Chun; Baba, Otto; Hwang, Pung Pung.

In: Journal of Experimental Biology, Vol. 210, No. 19, 01.10.2007, p. 3494-3504.

Research output: Contribution to journalArticle

Chang, Joshua Chia Hsi ; Wu, Su Mei ; Tseng, Yung-Che ; Lee, Yi Chun ; Baba, Otto ; Hwang, Pung Pung. / Regulation of glycogen metabolism in gills and liver of the euryhaline tilapia (Oreochromis mossambicus) during acclimation to seawater. In: Journal of Experimental Biology. 2007 ; Vol. 210, No. 19. pp. 3494-3504.
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AU - Baba, Otto

AU - Hwang, Pung Pung

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AB - Glucose, which plays a central role in providing energy for metabolism, is primarily stored as glycogen. The synthesis and degradation of glycogen are mainly initialized by glycogen synthase (GS) and glycogen Phosphorylase (GP), respectively. The present study aimed to examine the glycogen metabolism in fish liver and gills during acute exposure to seawater. In tilapia (Oreochromis mossambicus) gill, GP, GS and glycogen were immunocytochemically colocalized in a specific group of glycogen-rich (GR) cells, which are adjacent to the gill's main ionocytes, mitochondrion-rich (MR) cells. Na + /K + -ATPase activity in the gills, protein expression and/or activity of GP and GS and the glycogen content of the gills and liver were examined in tilapia after their acute transfer from freshwater (FW) to 25‰ seawater (SW). Gill Na + /K + -ATPase activity rapidly increased immediately after SW transfer. Glycogen content in both the gills and liver were significantly depleted after SW transfer, but the depletion occurred earlier in gills than in the liver. Gill GP activity and protein expression were upregulated 1-3 h posttransfer and eventually recovered to the normal level as determined in the control group. At the same time, GS protein expression was downregulated. Similar changes in liver GP and GS protein expression were also observed but they occurred later at 6-12 h post-transfer. In conclusion, GR cells are initially stimulated to provide prompt energy for neighboring MR cells that trigger ion-secretion mechanisms. Several hours later, the liver begins to degrade its glycogen stores for the subsequent energy supply.

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