Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA

Yi Chieh Chou; Chia I. Shih; Chih Chia Chiang; Chun Hua Hsu; Yi Chun Yeh

doi:10.1016/j.snb.2019.126717

Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA

Yi Chieh Chou, Chia I. Shih, Chih Chia Chiang, Chun Hua Hsu, Yi Chun Yeh^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.

Original language	English
Article number	126717
Journal	Sensors and Actuators, B: Chemical
Volume	297
DOIs	https://doi.org/10.1016/j.snb.2019.126717
Publication status	Published - 2019 Oct 15

Keywords

Catecholamine
Cell-based biosensor
Colorimetric biosensors
DOPA 4,5-Dioxygenase
L-DOPA

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2019.126717

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title = "Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA",

abstract = "As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.",

keywords = "Catecholamine, Cell-based biosensor, Colorimetric biosensors, DOPA 4,5-Dioxygenase, L-DOPA",

author = "Chou, {Yi Chieh} and Shih, {Chia I.} and Chiang, {Chih Chia} and Hsu, {Chun Hua} and Yeh, {Yi Chun}",

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year = "2019",

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day = "15",

doi = "10.1016/j.snb.2019.126717",

language = "English",

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T1 - Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA

AU - Chou, Yi Chieh

AU - Shih, Chia I.

AU - Chiang, Chih Chia

AU - Hsu, Chun Hua

AU - Yeh, Yi Chun

PY - 2019/10/15

Y1 - 2019/10/15

N2 - As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.

AB - As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.

KW - Catecholamine

KW - Cell-based biosensor

KW - Colorimetric biosensors

KW - DOPA 4,5-Dioxygenase

KW - L-DOPA

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JO - Sensors and Actuators, B: Chemical

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ER -

Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA

Abstract

Keywords

ASJC Scopus subject areas

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