Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA

Yi Chieh Chou, Chia I. Shih, Chih Chia Chiang, Chun Hua Hsu, Yi Chun Yeh

Research output: Contribution to journalArticle

Abstract

As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.

Original languageEnglish
Article number126717
JournalSensors and Actuators, B: Chemical
Volume297
DOIs
Publication statusPublished - 2019 Oct 15

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Keywords

  • Catecholamine
  • Cell-based biosensor
  • Colorimetric biosensors
  • DOPA 4,5-Dioxygenase
  • L-DOPA

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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