Rapid screening of Mycobacterium tuberculosis complex (MTBC) in clinical samples by a modular portable biosensor

Briliant Adhi Prabowo, Ying Feng Chang, Hsin Chih Lai, Azharul Alom, Parthasarathi Pal, Yu Ying Lee, Nan Fu Chiu, Koji Hatanaka, Li Chen Su, Kou Chen Liu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Mycobacterium tuberculosis complex (MTBC) is the well-known causative agents of tuberculosis (TB). Transmission of bacteria via airborne routes makes infection control difficult. Hence, rapid and accurate identification of TB from clinical sputum samples is essential for minimizing the spread of the disease. However, traditional bacterial identification methods for TB are time consuming and labor intensive. The aim of this study is to propose a straightforward and rapid screening platform for MTBC using a portable organic light-emitting diode- (OLED-) based surface plasmon resonance (SPR) biosensor integrated with a nested PCR technique. Experimentally, the limit of detection (LOD) for MTBC PCR products measurement was estimated to be around 63 pg/mL. In addition, the biosensor can successfully differentiate MTBC from other nontuberculous mycobacterium strains. In 600 clinical sputum specimens, the sensitivity and specificity were 96.6% (86/89) and 98.4% (503/511), respectively. These results indicate that the portable OLED-based SPR biosensor may be taken into account as a suitable and convenient tool employed in the remote area and developing country for diagnosing TB diseases.

Original languageEnglish
Pages (from-to)742-748
Number of pages7
JournalSensors and Actuators, B: Chemical
Publication statusPublished - 2018


  • DNA
  • Organic light-emitting diode (OLED)
  • Polymerase chain reaction (PCR)
  • Portable biosensor
  • Surface plasmon resonance (SPR)
  • Tuberculosis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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