TY - JOUR
T1 - Rapid screening of Mycobacterium tuberculosis complex (MTBC) in clinical samples by a modular portable biosensor
AU - Prabowo, Briliant Adhi
AU - Chang, Ying Feng
AU - Lai, Hsin Chih
AU - Alom, Azharul
AU - Pal, Parthasarathi
AU - Lee, Yu Ying
AU - Chiu, Nan Fu
AU - Hatanaka, Koji
AU - Su, Li Chen
AU - Liu, Kou Chen
N1 - Funding Information:
This research was supported by the Ministry of Science and Technology of Taiwan through grants from MOST 104-2112-M-034-001-MY2 and the National Research Program for Biopharmaceuticals, NSC102-2325-B182-005, NSC102-2325-B-182-007, and NRPB100-IDP-10082. Also, from Chang Gung Memorial Hospital project CMRPD190303 and CMRPD3E0231.
Publisher Copyright:
© 2017
PY - 2018
Y1 - 2018
N2 - Mycobacterium tuberculosis complex (MTBC) is the well-known causative agents of tuberculosis (TB). Transmission of bacteria via airborne routes makes infection control difficult. Hence, rapid and accurate identification of TB from clinical sputum samples is essential for minimizing the spread of the disease. However, traditional bacterial identification methods for TB are time consuming and labor intensive. The aim of this study is to propose a straightforward and rapid screening platform for MTBC using a portable organic light-emitting diode- (OLED-) based surface plasmon resonance (SPR) biosensor integrated with a nested PCR technique. Experimentally, the limit of detection (LOD) for MTBC PCR products measurement was estimated to be around 63 pg/mL. In addition, the biosensor can successfully differentiate MTBC from other nontuberculous mycobacterium strains. In 600 clinical sputum specimens, the sensitivity and specificity were 96.6% (86/89) and 98.4% (503/511), respectively. These results indicate that the portable OLED-based SPR biosensor may be taken into account as a suitable and convenient tool employed in the remote area and developing country for diagnosing TB diseases.
AB - Mycobacterium tuberculosis complex (MTBC) is the well-known causative agents of tuberculosis (TB). Transmission of bacteria via airborne routes makes infection control difficult. Hence, rapid and accurate identification of TB from clinical sputum samples is essential for minimizing the spread of the disease. However, traditional bacterial identification methods for TB are time consuming and labor intensive. The aim of this study is to propose a straightforward and rapid screening platform for MTBC using a portable organic light-emitting diode- (OLED-) based surface plasmon resonance (SPR) biosensor integrated with a nested PCR technique. Experimentally, the limit of detection (LOD) for MTBC PCR products measurement was estimated to be around 63 pg/mL. In addition, the biosensor can successfully differentiate MTBC from other nontuberculous mycobacterium strains. In 600 clinical sputum specimens, the sensitivity and specificity were 96.6% (86/89) and 98.4% (503/511), respectively. These results indicate that the portable OLED-based SPR biosensor may be taken into account as a suitable and convenient tool employed in the remote area and developing country for diagnosing TB diseases.
KW - DNA
KW - Organic light-emitting diode (OLED)
KW - Polymerase chain reaction (PCR)
KW - Portable biosensor
KW - Surface plasmon resonance (SPR)
KW - Tuberculosis
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U2 - 10.1016/j.snb.2017.07.102
DO - 10.1016/j.snb.2017.07.102
M3 - Article
AN - SCOPUS:85026384722
SN - 0925-4005
VL - 254
SP - 742
EP - 748
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -
