Rapid drug-screening and quantitation of 3,4-methylenedioxymethamphetamine in urine by MALDI-TOF mass spectrometry

An Kai Su, Ju Tsung Liu, Cheng-Huang Lin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A method for the rapid screening of the drugs, 3,4- methylenedioxymethamphetamine (MDMA), in urine by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described. In this method, α-cyano-4-hydroxycinnamic acid (CHCA) is used as the matrix for the MALDI samples. The identity of MDMA was confirmed by flight time of the fragmentation ([M + H]+ ion) using a mass calculation curve, the quantification of MDMA was achieved using stable-isotope-labeled MDMA (MDMA-D5) as the internal standard. For the analysis of MDMA/MDMA-D5-spiked urine samples, the calibration curve was linear in the range of 0.1-100 ppm with a coefficient of determination, r2, of 0.9993. This assay was used to determine MDMA in actual urine samples. The MALDI-TOFMS results were comparable to the GC/MS results. No significant bias is shown between the two methods by t-test at 95% confidence level. We conclude that MALDI-TOFMS can serve as a reliable and complementary method to GC/MS for use in forensic analysis.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalAnalytica Chimica Acta
Volume546
Issue number2
DOIs
Publication statusPublished - 2005 Aug 8

Fingerprint

N-Methyl-3,4-methylenedioxyamphetamine
Preclinical Drug Evaluations
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
urine
Mass spectrometry
Mass Spectrometry
Screening
drug
mass spectrometry
Urine
desorption
ionization
matrix
laser
Pharmaceutical Preparations
Ionization
Desorption
Lasers
fragmentation
stable isotope

Keywords

  • 3,4-Methylenedioxymethamphetamine (MDMA)
  • MALDI-TOF mass spectrometry
  • Rapid drug-screening
  • α-Cyano-4-hydroxycinnamic acid (CHCA)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Rapid drug-screening and quantitation of 3,4-methylenedioxymethamphetamine in urine by MALDI-TOF mass spectrometry. / Su, An Kai; Liu, Ju Tsung; Lin, Cheng-Huang.

In: Analytica Chimica Acta, Vol. 546, No. 2, 08.08.2005, p. 193-198.

Research output: Contribution to journalArticle

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