Quantitative relations of acoustic inertial cavitation with sonoporation and cell viability

Chun Yen Lai, Chia Hsuan Wu, Chia Chun Chen, Pai Chi Li

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Ultrasound-induced acoustic cavitation assists gene delivery, possibly by increasing the permeability of the cell membranes. How the cavitation dose is related to the sonoporation rate and the cell viability is still unknown and so this in vitro study quantitatively investigated the effects of cavitation induced by 1-MHz pulsed ultrasound waves and the contrast agent Levovist® (containing microbubbles when reconstituted by adding saline and shaken) on the delivery of short DNA-FITC molecules into HeLa cells. The concentrations of cells and DNA-FITC were 2 × 10 5 cells/mL and 40 μg/mL, respectively. The cavitation was quantified as the inertial cavitation dose (ICD), corresponding to the spectral broadband signal enhancement during microbubble destruction. The relations of ICD with sonoporation and cell viability were examined for various acoustic pressures (0.48-1.32 MPa), Levovist® concentrations (1.12 × 10 5 -1.12 × 10 7 bubbles/mL) and pulse durations (1-10 cycles). The linear regressions of the sonoporation rate versus ICD and the cell viability versus ICD were y = 28.67x + 10.71 (R 2 = 0.95) and z = -62.83x + 91.18 (R 2 = 0.84), respectively, where x is ICD, y is the sonoporation rate and z is the cell viability. These results show that the sonoporation rate and the cell viability are highly correlated with the ICD, indicating that sonoporation results may be potentially predicted using ICD. (E-mail: paichi@cc.ee.ntu.edu.tw).

Original languageEnglish
Pages (from-to)1931-1941
Number of pages11
JournalUltrasound in Medicine and Biology
Volume32
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1

Fingerprint

cavitation flow
viability
Acoustics
Cell Survival
acoustics
Microbubbles
Fluorescein-5-isothiocyanate
dosage
Cell Membrane Permeability
DNA
HeLa Cells
Contrast Media
Linear Models
delivery
deoxyribonucleic acid
Pressure
cells
Genes
genes
destruction

Keywords

  • Cavitation
  • HeLa
  • Inertial cavitation dose
  • Levovist®
  • Sonoporation
  • Ultrasound

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Biophysics
  • Acoustics and Ultrasonics

Cite this

Quantitative relations of acoustic inertial cavitation with sonoporation and cell viability. / Lai, Chun Yen; Wu, Chia Hsuan; Chen, Chia Chun; Li, Pai Chi.

In: Ultrasound in Medicine and Biology, Vol. 32, No. 12, 01.12.2006, p. 1931-1941.

Research output: Contribution to journalArticle

@article{11552efb5d8b4f8eabb8595046456fb9,
title = "Quantitative relations of acoustic inertial cavitation with sonoporation and cell viability",
abstract = "Ultrasound-induced acoustic cavitation assists gene delivery, possibly by increasing the permeability of the cell membranes. How the cavitation dose is related to the sonoporation rate and the cell viability is still unknown and so this in vitro study quantitatively investigated the effects of cavitation induced by 1-MHz pulsed ultrasound waves and the contrast agent Levovist{\circledR} (containing microbubbles when reconstituted by adding saline and shaken) on the delivery of short DNA-FITC molecules into HeLa cells. The concentrations of cells and DNA-FITC were 2 × 10 5 cells/mL and 40 μg/mL, respectively. The cavitation was quantified as the inertial cavitation dose (ICD), corresponding to the spectral broadband signal enhancement during microbubble destruction. The relations of ICD with sonoporation and cell viability were examined for various acoustic pressures (0.48-1.32 MPa), Levovist{\circledR} concentrations (1.12 × 10 5 -1.12 × 10 7 bubbles/mL) and pulse durations (1-10 cycles). The linear regressions of the sonoporation rate versus ICD and the cell viability versus ICD were y = 28.67x + 10.71 (R 2 = 0.95) and z = -62.83x + 91.18 (R 2 = 0.84), respectively, where x is ICD, y is the sonoporation rate and z is the cell viability. These results show that the sonoporation rate and the cell viability are highly correlated with the ICD, indicating that sonoporation results may be potentially predicted using ICD. (E-mail: paichi@cc.ee.ntu.edu.tw).",
keywords = "Cavitation, HeLa, Inertial cavitation dose, Levovist{\circledR}, Sonoporation, Ultrasound",
author = "Lai, {Chun Yen} and Wu, {Chia Hsuan} and Chen, {Chia Chun} and Li, {Pai Chi}",
year = "2006",
month = "12",
day = "1",
doi = "10.1016/j.ultrasmedbio.2006.06.020",
language = "English",
volume = "32",
pages = "1931--1941",
journal = "Ultrasound in Medicine and Biology",
issn = "0301-5629",
publisher = "Elsevier USA",
number = "12",

}

TY - JOUR

T1 - Quantitative relations of acoustic inertial cavitation with sonoporation and cell viability

AU - Lai, Chun Yen

AU - Wu, Chia Hsuan

AU - Chen, Chia Chun

AU - Li, Pai Chi

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Ultrasound-induced acoustic cavitation assists gene delivery, possibly by increasing the permeability of the cell membranes. How the cavitation dose is related to the sonoporation rate and the cell viability is still unknown and so this in vitro study quantitatively investigated the effects of cavitation induced by 1-MHz pulsed ultrasound waves and the contrast agent Levovist® (containing microbubbles when reconstituted by adding saline and shaken) on the delivery of short DNA-FITC molecules into HeLa cells. The concentrations of cells and DNA-FITC were 2 × 10 5 cells/mL and 40 μg/mL, respectively. The cavitation was quantified as the inertial cavitation dose (ICD), corresponding to the spectral broadband signal enhancement during microbubble destruction. The relations of ICD with sonoporation and cell viability were examined for various acoustic pressures (0.48-1.32 MPa), Levovist® concentrations (1.12 × 10 5 -1.12 × 10 7 bubbles/mL) and pulse durations (1-10 cycles). The linear regressions of the sonoporation rate versus ICD and the cell viability versus ICD were y = 28.67x + 10.71 (R 2 = 0.95) and z = -62.83x + 91.18 (R 2 = 0.84), respectively, where x is ICD, y is the sonoporation rate and z is the cell viability. These results show that the sonoporation rate and the cell viability are highly correlated with the ICD, indicating that sonoporation results may be potentially predicted using ICD. (E-mail: paichi@cc.ee.ntu.edu.tw).

AB - Ultrasound-induced acoustic cavitation assists gene delivery, possibly by increasing the permeability of the cell membranes. How the cavitation dose is related to the sonoporation rate and the cell viability is still unknown and so this in vitro study quantitatively investigated the effects of cavitation induced by 1-MHz pulsed ultrasound waves and the contrast agent Levovist® (containing microbubbles when reconstituted by adding saline and shaken) on the delivery of short DNA-FITC molecules into HeLa cells. The concentrations of cells and DNA-FITC were 2 × 10 5 cells/mL and 40 μg/mL, respectively. The cavitation was quantified as the inertial cavitation dose (ICD), corresponding to the spectral broadband signal enhancement during microbubble destruction. The relations of ICD with sonoporation and cell viability were examined for various acoustic pressures (0.48-1.32 MPa), Levovist® concentrations (1.12 × 10 5 -1.12 × 10 7 bubbles/mL) and pulse durations (1-10 cycles). The linear regressions of the sonoporation rate versus ICD and the cell viability versus ICD were y = 28.67x + 10.71 (R 2 = 0.95) and z = -62.83x + 91.18 (R 2 = 0.84), respectively, where x is ICD, y is the sonoporation rate and z is the cell viability. These results show that the sonoporation rate and the cell viability are highly correlated with the ICD, indicating that sonoporation results may be potentially predicted using ICD. (E-mail: paichi@cc.ee.ntu.edu.tw).

KW - Cavitation

KW - HeLa

KW - Inertial cavitation dose

KW - Levovist®

KW - Sonoporation

KW - Ultrasound

UR - http://www.scopus.com/inward/record.url?scp=33845341801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845341801&partnerID=8YFLogxK

U2 - 10.1016/j.ultrasmedbio.2006.06.020

DO - 10.1016/j.ultrasmedbio.2006.06.020

M3 - Article

C2 - 17169705

AN - SCOPUS:33845341801

VL - 32

SP - 1931

EP - 1941

JO - Ultrasound in Medicine and Biology

JF - Ultrasound in Medicine and Biology

SN - 0301-5629

IS - 12

ER -