Quantitative relations between acoustic inertial cavitation and gene transfection rate/cell viability

Chun Yen Lai, Chia Hsuan Wu, Chia Chun Chen, Pai Chi Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Ultrasound has been utilized to assist gene transfection. In combination with ultrasound, microbubble-based contrast agents have also been used to enhance the transfection efficiency by inducing acoustic inertial cavitation. However, the relations between inertial cavitation and gene transfection rate/cell viability are still unknown. It is the primary purpose of this study to examine the relations experimentally and quantitatively. A commercial contrast agent Levovist® was used, and the cavitation was represented by the parameter ICD (inertial cavitation dose). The relations between ICD and gene delivery rate/cell viability were studied under various acoustic pressures, microbubble concentrations and pulse durations. Concentration of cells (HeLa cells) and DNA (short DNA-FITC molecules) were 2×10 5 cells/mL and 40 μg/mL, respectively. Linear regression was performed on the delivery rate versus ICD and the cell viability versus ICD. For the delivery, y=28.67x+10.71 (x: ICD, y: delivery rate) was obtained (R 2 =0.95). On the other hand, y=-62.83x+91.18 (x: ICD, y: cell viability) was obtained (R 2 =0.84) for cell viability. The results show high correlations between delivery rate/cell viability and ICD.

Original languageEnglish
Title of host publication2005 IEEE Ultrasonics Symposium
Pages1720-1723
Number of pages4
DOIs
Publication statusPublished - 2005 Dec 1
Event2005 IEEE Ultrasonics Symposium - Rotterdam, Netherlands
Duration: 2005 Sep 182005 Sep 21

Publication series

NameProceedings - IEEE Ultrasonics Symposium
Volume3
ISSN (Print)1051-0117

Other

Other2005 IEEE Ultrasonics Symposium
CountryNetherlands
CityRotterdam
Period05/9/1805/9/21

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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