Quantitative analysis of multiple urinary biomarkers of carcinoid tumors through gold-nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry

Tsung Rong Kuo, Jinn Shiun Chen, Yu Chen Chiu, Chia Yi Tsai, Cho Chun Hu, Chia Chun Chen

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A simple technique for quantitative analysis of four urinary biomarkers, tryptophan (TRP), 5-hydroxytryptophan (5-HTP), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) of carcinoid tumors is developed using gold nanoparticles as the assisted matrix in surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF MS). The optimal SALDI conditions for the efficient ionization of those biomarkers are systematically explored by the adjustments of the concentrations of gold nanoparticles and internal standards. The mass spectra with strong signals and minimal background noise are obtained using 1-naphthaleneacetamide (NAD) as the internal standard. The calibration curves of the biomarker concentrations are determined using SALDI-TOF MS and the high linearity is obtained in all samples. For future clinical testing, multiplexed detection of those biomarkers in the urine samples of healthy males is performed. The successful quantitative detections of TRP, 5-HTP, 5-HT and 5-HIAA indicate that our technique provided great potentials to be developed a simple and rapid platform for the tumor biomarker detections.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalAnalytica Chimica Acta
Volume699
Issue number1
DOIs
Publication statusPublished - 2011 Aug 5

Keywords

  • 5-Hydroxyindole acetic acid
  • Gold nanoparticles
  • Multiplexed assay
  • Surface-assisted laser desorption/ionization
  • Urinary biomarkers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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