Quantification of anthocyanins in blueberries (Vaccinium spp.) by modified QuEChERS and liquid chromatography-mass spectrometry

Lun Chi Yang, Shih Hsin Hsu, Yun Yu Meng, Sung Fang Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Anthocyanins are among the most extensively investigated class of bioflavonoids in plant and fruit products due to their widespread presence in nature and their antioxidant activity. A method based on a modified quick, easy, cheap, effective, rugged, and safe method (QuEChERS) was developed for the determination of six common types of anthocyanins in blueberries using liquid chromatography–tandem mass spectrometry (LC–MS/MS). The QuEChERS procedure was optimized in the dispersive solid-phase extraction (d-SPE) for obtaining the targeted anthocyanins. This method provided the quantitative ranges between 2.5 and 1,000 μg L−1 (R2 > 0.9974) for the target analytes with satisfactory recoveries and precisions. The developed method was successfully applied to the analysis of anthocyanins in blueberries from different countries and the total anthocyanins concentrations were in the range from 926.5 to 2074.6 μg kg−1. This proposed method provided an efficient and sensitive approach for the qualitative and quantitative determination of the common anthocyanins in blueberry samples.

Original languageEnglish
Pages (from-to)1070-1078
Number of pages9
JournalJournal of the Chinese Chemical Society
Volume69
Issue number7
DOIs
Publication statusPublished - 2022 Jul

Keywords

  • Anthocyanins
  • LC–MS/MS
  • QuEChERS
  • blueberry
  • quantitative

ASJC Scopus subject areas

  • Chemistry(all)

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