Protein Attachment on Nanodiamonds

Chung Lun Lin, Cheng Huang Lin, Huan Cheng Chang, Meng Chih Su

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A recent advance in nanotechnology is the scale-up production of small and nonaggregated diamond nanoparticles suitable for biological applications. Using detonation nanodiamonds (NDs) with an average diameter of ∼4 nm as the adsorbents, we have studied the static attachment of three proteins (myoglobin, bovine serum albumin, and insulin) onto the nanoparticles by optical spectroscopy, mass spectrometry, and dynamic light scattering, and electrophoretic zeta potential measurements. Results show that the protein surface coverage is predominantly determined by the competition between protein-protein and protein-ND interactions, giving each protein a unique and characteristic structural configuration in its own complex. Specifically, both myoglobin and bovine serum albumin show a Langmuir-type adsorption behavior, forming 1:1 complexes at saturation, whereas insulin folds into a tightly bound multimer before adsorption. The markedly different adsorption patterns appear to be independent of the protein concentration and are closely related to the affinity of the individual proteins for the NDs. The present study provides a fundamental understanding for the use of NDs as a platform for nanomedical drug delivery.

Original languageEnglish
Pages (from-to)7704-7711
Number of pages8
JournalJournal of Physical Chemistry A
Volume119
Issue number28
DOIs
Publication statusPublished - 2015 Jul 16

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Nanodiamonds
attachment
proteins
Proteins
Adsorption
Myoglobin
insulin
myoglobin
Bovine Serum Albumin
Nanoparticles
albumins
serums
adsorption
Insulin
Nanotechnology
Diamond
nanoparticles
Dynamic light scattering
Zeta potential
Detonation

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Protein Attachment on Nanodiamonds. / Lin, Chung Lun; Lin, Cheng Huang; Chang, Huan Cheng; Su, Meng Chih.

In: Journal of Physical Chemistry A, Vol. 119, No. 28, 16.07.2015, p. 7704-7711.

Research output: Contribution to journalArticle

Lin, Chung Lun ; Lin, Cheng Huang ; Chang, Huan Cheng ; Su, Meng Chih. / Protein Attachment on Nanodiamonds. In: Journal of Physical Chemistry A. 2015 ; Vol. 119, No. 28. pp. 7704-7711.
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