Protective effect of: Ruellia tuberosa L. extracts against abnormal expression of hepatic detoxification enzymes in diabetic rats

Wen Chang Chang, Da Wei Huang, Jou An Chen, Yu Fang Chang, James Swi-Bea Wu, Szu Chuan Shen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ruellia tuberosa L. (RTL) has been used as a folk medicine for curing diabetes mellitus in East Asia decades. This study investigated the effect of RTL on hepatic detoxification enzyme expression in diabetic rats. Male Wistar rats were fed a high fat diet (HFD) and intraperitoneally injected with streptozotocin (STZ) to induce diabetes. Subsequently, rats received oral administrations of 100 or 400 mg kg-1 body weight RTL extract, in either water (RTLW) or ethanol (RTLE), once a day for 4 weeks. The real-time PCR analyses showed that abnormality of hepatic phase I and II detoxification enzyme expression was observed in diabetic rats. However, both RTLW and RTLE significantly normalized the expression of hepatic phase I detoxification enzymes such as CYP 2E1, and expression of phase II detoxification enzymes such as UGT 1A7 and GST M1 in diabetic rats. Furthermore, we found that fasting serum glucose, hemoglobin A1C (HbA1C) and the area under the curve of oral glucose tolerance test (AUCOGTT) levels were significantly reduced in both RTLW and RTLE treated diabetic rats. Moreover, both RTLW and RTLE significantly increased the activity of hepatic anti-oxidative enzymes such as superoxide dismutase (SOD) in diabetic rats. The present study suggests that RTL may ameliorate abnormal hepatic detoxification function via alleviating hyperglycemia and enhancing hepatic antioxidant capacity in HFD/STZ-induced diabetic rats.

Original languageEnglish
Pages (from-to)21596-21605
Number of pages10
JournalRSC Advances
Volume8
Issue number38
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Detoxification
Rats
Enzymes
Ethanol
Nutrition
Streptozocin
Medical problems
Oils and fats
Glucose
Fats
Cytochrome P-450 CYP2E1
Hemoglobin
Antioxidants
Superoxide Dismutase
Medicine
Curing
Hemoglobins
Water

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Protective effect of : Ruellia tuberosa L. extracts against abnormal expression of hepatic detoxification enzymes in diabetic rats. / Chang, Wen Chang; Huang, Da Wei; Chen, Jou An; Chang, Yu Fang; Swi-Bea Wu, James; Shen, Szu Chuan.

In: RSC Advances, Vol. 8, No. 38, 01.01.2018, p. 21596-21605.

Research output: Contribution to journalArticle

Chang, Wen Chang ; Huang, Da Wei ; Chen, Jou An ; Chang, Yu Fang ; Swi-Bea Wu, James ; Shen, Szu Chuan. / Protective effect of : Ruellia tuberosa L. extracts against abnormal expression of hepatic detoxification enzymes in diabetic rats. In: RSC Advances. 2018 ; Vol. 8, No. 38. pp. 21596-21605.
@article{5f25ed3a98a74f8dad17fc5a2583c30c,
title = "Protective effect of: Ruellia tuberosa L. extracts against abnormal expression of hepatic detoxification enzymes in diabetic rats",
abstract = "Ruellia tuberosa L. (RTL) has been used as a folk medicine for curing diabetes mellitus in East Asia decades. This study investigated the effect of RTL on hepatic detoxification enzyme expression in diabetic rats. Male Wistar rats were fed a high fat diet (HFD) and intraperitoneally injected with streptozotocin (STZ) to induce diabetes. Subsequently, rats received oral administrations of 100 or 400 mg kg-1 body weight RTL extract, in either water (RTLW) or ethanol (RTLE), once a day for 4 weeks. The real-time PCR analyses showed that abnormality of hepatic phase I and II detoxification enzyme expression was observed in diabetic rats. However, both RTLW and RTLE significantly normalized the expression of hepatic phase I detoxification enzymes such as CYP 2E1, and expression of phase II detoxification enzymes such as UGT 1A7 and GST M1 in diabetic rats. Furthermore, we found that fasting serum glucose, hemoglobin A1C (HbA1C) and the area under the curve of oral glucose tolerance test (AUCOGTT) levels were significantly reduced in both RTLW and RTLE treated diabetic rats. Moreover, both RTLW and RTLE significantly increased the activity of hepatic anti-oxidative enzymes such as superoxide dismutase (SOD) in diabetic rats. The present study suggests that RTL may ameliorate abnormal hepatic detoxification function via alleviating hyperglycemia and enhancing hepatic antioxidant capacity in HFD/STZ-induced diabetic rats.",
author = "Chang, {Wen Chang} and Huang, {Da Wei} and Chen, {Jou An} and Chang, {Yu Fang} and {Swi-Bea Wu}, James and Shen, {Szu Chuan}",
year = "2018",
month = "1",
day = "1",
doi = "10.1039/c8ra03321h",
language = "English",
volume = "8",
pages = "21596--21605",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "38",

}

TY - JOUR

T1 - Protective effect of

T2 - Ruellia tuberosa L. extracts against abnormal expression of hepatic detoxification enzymes in diabetic rats

AU - Chang, Wen Chang

AU - Huang, Da Wei

AU - Chen, Jou An

AU - Chang, Yu Fang

AU - Swi-Bea Wu, James

AU - Shen, Szu Chuan

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Ruellia tuberosa L. (RTL) has been used as a folk medicine for curing diabetes mellitus in East Asia decades. This study investigated the effect of RTL on hepatic detoxification enzyme expression in diabetic rats. Male Wistar rats were fed a high fat diet (HFD) and intraperitoneally injected with streptozotocin (STZ) to induce diabetes. Subsequently, rats received oral administrations of 100 or 400 mg kg-1 body weight RTL extract, in either water (RTLW) or ethanol (RTLE), once a day for 4 weeks. The real-time PCR analyses showed that abnormality of hepatic phase I and II detoxification enzyme expression was observed in diabetic rats. However, both RTLW and RTLE significantly normalized the expression of hepatic phase I detoxification enzymes such as CYP 2E1, and expression of phase II detoxification enzymes such as UGT 1A7 and GST M1 in diabetic rats. Furthermore, we found that fasting serum glucose, hemoglobin A1C (HbA1C) and the area under the curve of oral glucose tolerance test (AUCOGTT) levels were significantly reduced in both RTLW and RTLE treated diabetic rats. Moreover, both RTLW and RTLE significantly increased the activity of hepatic anti-oxidative enzymes such as superoxide dismutase (SOD) in diabetic rats. The present study suggests that RTL may ameliorate abnormal hepatic detoxification function via alleviating hyperglycemia and enhancing hepatic antioxidant capacity in HFD/STZ-induced diabetic rats.

AB - Ruellia tuberosa L. (RTL) has been used as a folk medicine for curing diabetes mellitus in East Asia decades. This study investigated the effect of RTL on hepatic detoxification enzyme expression in diabetic rats. Male Wistar rats were fed a high fat diet (HFD) and intraperitoneally injected with streptozotocin (STZ) to induce diabetes. Subsequently, rats received oral administrations of 100 or 400 mg kg-1 body weight RTL extract, in either water (RTLW) or ethanol (RTLE), once a day for 4 weeks. The real-time PCR analyses showed that abnormality of hepatic phase I and II detoxification enzyme expression was observed in diabetic rats. However, both RTLW and RTLE significantly normalized the expression of hepatic phase I detoxification enzymes such as CYP 2E1, and expression of phase II detoxification enzymes such as UGT 1A7 and GST M1 in diabetic rats. Furthermore, we found that fasting serum glucose, hemoglobin A1C (HbA1C) and the area under the curve of oral glucose tolerance test (AUCOGTT) levels were significantly reduced in both RTLW and RTLE treated diabetic rats. Moreover, both RTLW and RTLE significantly increased the activity of hepatic anti-oxidative enzymes such as superoxide dismutase (SOD) in diabetic rats. The present study suggests that RTL may ameliorate abnormal hepatic detoxification function via alleviating hyperglycemia and enhancing hepatic antioxidant capacity in HFD/STZ-induced diabetic rats.

UR - http://www.scopus.com/inward/record.url?scp=85048777270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048777270&partnerID=8YFLogxK

U2 - 10.1039/c8ra03321h

DO - 10.1039/c8ra03321h

M3 - Article

AN - SCOPUS:85048777270

VL - 8

SP - 21596

EP - 21605

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 38

ER -