Protection "outside the box" (skeletal remote preconditioning) in rat model is triggered by free radical pathway

Yih Sharng Chen, Chiang-Ting Chien, Ming Chieh Ma, Yung Zu Tseng, Fang Yue Lin, Shoei Shen Wang, Chau Fong Chen

Research output: Contribution to journalArticle

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Abstract

Background. Remote preconditioning (RPC) for myocardial protection had been demonstrated in several organs, such as the kidney and mesentery artery. The aim of study was to investigate the effect of skeletal ischemia/reperfusion on coronary artery occlusion-induced myocardial infarction and to investigate the role of the free radicals. Material and methods. RPC was performed in rats by a repeated four-cycle 10-min ischemia-reperfusion of femoral artery. Four experimental groups were included: I, sham group; II, RPC only; III, infarction only; and IV, which incorporated both RPC and infarction. A chemiluminescence study showed significant elevation of free radicals in groups with RPC, and pretreated mercaptopropionyl-glycine (MPG), a free radical scavenger, abolished the production of free radicals. Results. The infarct size was significantly reduced for group IV (24.7 ± 8.8%) compared with group III (51.4 ± 9.1%; P < 0.001), and the effect was abolished by pretreatment with MPG (49.2 ± 6.3% in MPG + III versus 50.1 ± 8.2% in MPG + IV; P > 0.05). Cardiac enzymes also revealed significant decrease in the level for group IV compared with group III, and the protective effect could be abolished by MPG. Western blotting of heat shock protein (HSP) revealed that consistent elevation of HSP 25 and 70 in groups II, III, and IV, and the elevation can be abrogated by pretreatment with MPG. The expression of the antioxidant enzymes, Mn-superoxidase dismutase and glutathione peroxidase, in the area of risk were consistently elevated in groups II, III, and IV, similar to HSP. Conclusions. The skeletal RPC in rats can produce a protective effect in an infarction model that may be triggered through free radical pathway, and the protective effect was associated with HSP and antioxidant enzymes.

Original languageEnglish
Pages (from-to)92-101
Number of pages10
JournalJournal of Surgical Research
Volume126
Issue number1
DOIs
Publication statusPublished - 2005 Jun 1

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Free Radicals
Heat-Shock Proteins
Glycine
Infarction
Reperfusion
Enzymes
Myocardial Ischemic Preconditioning
Ischemia
Antioxidants
Free Radical Scavengers
HSP70 Heat-Shock Proteins
Mesentery
Coronary Occlusion
Femoral Artery
Glutathione Peroxidase
Luminescence
Coronary Vessels
Arteries
Western Blotting
Myocardial Infarction

Keywords

  • Free radical
  • Heat shock protein
  • Infarct size
  • Preconditioning
  • Remote
  • Skeletal muscle

ASJC Scopus subject areas

  • Surgery

Cite this

Protection "outside the box" (skeletal remote preconditioning) in rat model is triggered by free radical pathway. / Chen, Yih Sharng; Chien, Chiang-Ting; Ma, Ming Chieh; Tseng, Yung Zu; Lin, Fang Yue; Wang, Shoei Shen; Chen, Chau Fong.

In: Journal of Surgical Research, Vol. 126, No. 1, 01.06.2005, p. 92-101.

Research output: Contribution to journalArticle

Chen, Yih Sharng ; Chien, Chiang-Ting ; Ma, Ming Chieh ; Tseng, Yung Zu ; Lin, Fang Yue ; Wang, Shoei Shen ; Chen, Chau Fong. / Protection "outside the box" (skeletal remote preconditioning) in rat model is triggered by free radical pathway. In: Journal of Surgical Research. 2005 ; Vol. 126, No. 1. pp. 92-101.
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abstract = "Background. Remote preconditioning (RPC) for myocardial protection had been demonstrated in several organs, such as the kidney and mesentery artery. The aim of study was to investigate the effect of skeletal ischemia/reperfusion on coronary artery occlusion-induced myocardial infarction and to investigate the role of the free radicals. Material and methods. RPC was performed in rats by a repeated four-cycle 10-min ischemia-reperfusion of femoral artery. Four experimental groups were included: I, sham group; II, RPC only; III, infarction only; and IV, which incorporated both RPC and infarction. A chemiluminescence study showed significant elevation of free radicals in groups with RPC, and pretreated mercaptopropionyl-glycine (MPG), a free radical scavenger, abolished the production of free radicals. Results. The infarct size was significantly reduced for group IV (24.7 ± 8.8{\%}) compared with group III (51.4 ± 9.1{\%}; P < 0.001), and the effect was abolished by pretreatment with MPG (49.2 ± 6.3{\%} in MPG + III versus 50.1 ± 8.2{\%} in MPG + IV; P > 0.05). Cardiac enzymes also revealed significant decrease in the level for group IV compared with group III, and the protective effect could be abolished by MPG. Western blotting of heat shock protein (HSP) revealed that consistent elevation of HSP 25 and 70 in groups II, III, and IV, and the elevation can be abrogated by pretreatment with MPG. The expression of the antioxidant enzymes, Mn-superoxidase dismutase and glutathione peroxidase, in the area of risk were consistently elevated in groups II, III, and IV, similar to HSP. Conclusions. The skeletal RPC in rats can produce a protective effect in an infarction model that may be triggered through free radical pathway, and the protective effect was associated with HSP and antioxidant enzymes.",
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T1 - Protection "outside the box" (skeletal remote preconditioning) in rat model is triggered by free radical pathway

AU - Chen, Yih Sharng

AU - Chien, Chiang-Ting

AU - Ma, Ming Chieh

AU - Tseng, Yung Zu

AU - Lin, Fang Yue

AU - Wang, Shoei Shen

AU - Chen, Chau Fong

PY - 2005/6/1

Y1 - 2005/6/1

N2 - Background. Remote preconditioning (RPC) for myocardial protection had been demonstrated in several organs, such as the kidney and mesentery artery. The aim of study was to investigate the effect of skeletal ischemia/reperfusion on coronary artery occlusion-induced myocardial infarction and to investigate the role of the free radicals. Material and methods. RPC was performed in rats by a repeated four-cycle 10-min ischemia-reperfusion of femoral artery. Four experimental groups were included: I, sham group; II, RPC only; III, infarction only; and IV, which incorporated both RPC and infarction. A chemiluminescence study showed significant elevation of free radicals in groups with RPC, and pretreated mercaptopropionyl-glycine (MPG), a free radical scavenger, abolished the production of free radicals. Results. The infarct size was significantly reduced for group IV (24.7 ± 8.8%) compared with group III (51.4 ± 9.1%; P < 0.001), and the effect was abolished by pretreatment with MPG (49.2 ± 6.3% in MPG + III versus 50.1 ± 8.2% in MPG + IV; P > 0.05). Cardiac enzymes also revealed significant decrease in the level for group IV compared with group III, and the protective effect could be abolished by MPG. Western blotting of heat shock protein (HSP) revealed that consistent elevation of HSP 25 and 70 in groups II, III, and IV, and the elevation can be abrogated by pretreatment with MPG. The expression of the antioxidant enzymes, Mn-superoxidase dismutase and glutathione peroxidase, in the area of risk were consistently elevated in groups II, III, and IV, similar to HSP. Conclusions. The skeletal RPC in rats can produce a protective effect in an infarction model that may be triggered through free radical pathway, and the protective effect was associated with HSP and antioxidant enzymes.

AB - Background. Remote preconditioning (RPC) for myocardial protection had been demonstrated in several organs, such as the kidney and mesentery artery. The aim of study was to investigate the effect of skeletal ischemia/reperfusion on coronary artery occlusion-induced myocardial infarction and to investigate the role of the free radicals. Material and methods. RPC was performed in rats by a repeated four-cycle 10-min ischemia-reperfusion of femoral artery. Four experimental groups were included: I, sham group; II, RPC only; III, infarction only; and IV, which incorporated both RPC and infarction. A chemiluminescence study showed significant elevation of free radicals in groups with RPC, and pretreated mercaptopropionyl-glycine (MPG), a free radical scavenger, abolished the production of free radicals. Results. The infarct size was significantly reduced for group IV (24.7 ± 8.8%) compared with group III (51.4 ± 9.1%; P < 0.001), and the effect was abolished by pretreatment with MPG (49.2 ± 6.3% in MPG + III versus 50.1 ± 8.2% in MPG + IV; P > 0.05). Cardiac enzymes also revealed significant decrease in the level for group IV compared with group III, and the protective effect could be abolished by MPG. Western blotting of heat shock protein (HSP) revealed that consistent elevation of HSP 25 and 70 in groups II, III, and IV, and the elevation can be abrogated by pretreatment with MPG. The expression of the antioxidant enzymes, Mn-superoxidase dismutase and glutathione peroxidase, in the area of risk were consistently elevated in groups II, III, and IV, similar to HSP. Conclusions. The skeletal RPC in rats can produce a protective effect in an infarction model that may be triggered through free radical pathway, and the protective effect was associated with HSP and antioxidant enzymes.

KW - Free radical

KW - Heat shock protein

KW - Infarct size

KW - Preconditioning

KW - Remote

KW - Skeletal muscle

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