Protection of ischemic preconditioning on renal neural function in rats with acute renal failure

Ming Shiou Wu, Chiang-Ting Chien, Ming Chieh Ma, Chau Fong Chen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We tested whether tolerance induced by ischemic preconditioning (IPC) in kidneys was related to renal nerves. Experimental acute renal failure (ARF) in a rat model was induced for 45 min of left renal arterial occlusion (RAO), followed by 6 or 24 h of reperfusion (ischemic reperfusion (I/R) group). The episode of IPC was four cycles of 4 min of RAO at 11 min intervals and then the I/R injury was treated as above (IPC-I/R group). After 6 h of reperfusion, polyuria was found in the I/R group associated with an enhancement of afferent renal nerve activity (ARNA) and a reflexive decrease in efferent renal nerve activity (ERNA). Changes in nerve responses were related with a reduction in neutral endopeptidase (NEP) activity and an increased release of substance P (SP). After 24 h of reperfusion, the I/R group showed oliguria which was associated with a lower ARNA, hyperactivity of ERNA and a nine-fold increase in SP release due to a further 52% loss in NEP activity. Prior IPC treatment did not affect the changed ischemia-induced excretory and nervous activity patterns during the first 6 h of reperfusion, but normalized both responses in the kidneys 24 h after ischemia. The IPC-mediated protection in oliguric ARF was related to the preservation of NEP activity to only 25% loss that caused an increase of SP amounts of only three-fold and a minor change in neurokinin 1 receptor (NK-1R) activities. Finally, both excretory and sensory responses in oliguric ARF after saline loading were significantly ameliorated by IPC. We conclude that IPC results in preservation of the renal sensory response in postischemic kidneys and has a beneficial effect on controlling efferent renal sympathetic nerve activity and excretion of solutes and water.

Original languageEnglish
Pages (from-to)365-375
Number of pages11
JournalChinese Journal of Physiology
Volume52
Issue numberSUPPL. 5
DOIs
Publication statusPublished - 2009 Jan 1

Fingerprint

Ischemic Preconditioning
Acute Kidney Injury
Kidney
Reperfusion
Neprilysin
Substance P
Ischemia
Oliguria
Neurokinin-1 Receptors
Polyuria
Reperfusion Injury

Keywords

  • Acute renal failure
  • Neurokinin 1 receptor
  • Neutral endopeptidase
  • Preconditioned kidney
  • Renal afferent nerves
  • Substance P

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Protection of ischemic preconditioning on renal neural function in rats with acute renal failure. / Wu, Ming Shiou; Chien, Chiang-Ting; Ma, Ming Chieh; Chen, Chau Fong.

In: Chinese Journal of Physiology, Vol. 52, No. SUPPL. 5, 01.01.2009, p. 365-375.

Research output: Contribution to journalArticle

Wu, Ming Shiou ; Chien, Chiang-Ting ; Ma, Ming Chieh ; Chen, Chau Fong. / Protection of ischemic preconditioning on renal neural function in rats with acute renal failure. In: Chinese Journal of Physiology. 2009 ; Vol. 52, No. SUPPL. 5. pp. 365-375.
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