Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles

H. T. Chan; Y. Y. Do; P. L. Huang; P. L. Chien; T. S. Chan; R. S. Liu; C. Y. Huang; S. Y. Yang; H. E. Horng

doi:10.1016/j.jmmm.2006.01.126

Preparation and properties of bio-compatible magnetic Fe₃O₄ nanoparticles

H. T. Chan, Y. Y. Do, P. L. Huang^*, P. L. Chien, T. S. Chan, R. S. Liu, C. Y. Huang, S. Y. Yang, H. E. Horng

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe₃O₄) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin.

Original language	English
Pages (from-to)	e415-e417
Journal	Journal of Magnetism and Magnetic Materials
Volume	304
Issue number	1
DOIs	https://doi.org/10.1016/j.jmmm.2006.01.126
Publication status	Published - 2006 Sept

Keywords

Avidin
Bio-compatible nanoparticles
Biotinylated alkaline phosphatase
Hybridization

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

Access to Document

10.1016/j.jmmm.2006.01.126

Cite this

@article{ae0c73ce1ef2447f9f283e2afbe1e5b9,

title = "Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles",

abstract = "In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin.",

keywords = "Avidin, Bio-compatible nanoparticles, Biotinylated alkaline phosphatase, Hybridization",

author = "Chan, {H. T.} and Do, {Y. Y.} and Huang, {P. L.} and Chien, {P. L.} and Chan, {T. S.} and Liu, {R. S.} and Huang, {C. Y.} and Yang, {S. Y.} and Horng, {H. E.}",

note = "Funding Information: We thank the financial support from the Ministry Economic Affairs of Taiwan under the Grant number 94-EC-17-A-08-S1-0006. Research in P.L. Huang's laboratory relating to this work is supported by the Council of Agriculture, Republic of China (Grant Nos. 94AS-5.1.3-ST-a4 and 94AS-5.2.1-AD-U2).",

year = "2006",

month = sep,

doi = "10.1016/j.jmmm.2006.01.126",

language = "English",

volume = "304",

pages = "e415--e417",

journal = "Journal of Magnetism and Magnetic Materials",

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T1 - Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles

AU - Chan, H. T.

AU - Do, Y. Y.

AU - Huang, P. L.

AU - Chien, P. L.

AU - Chan, T. S.

AU - Liu, R. S.

AU - Huang, C. Y.

AU - Yang, S. Y.

AU - Horng, H. E.

N1 - Funding Information: We thank the financial support from the Ministry Economic Affairs of Taiwan under the Grant number 94-EC-17-A-08-S1-0006. Research in P.L. Huang's laboratory relating to this work is supported by the Council of Agriculture, Republic of China (Grant Nos. 94AS-5.1.3-ST-a4 and 94AS-5.2.1-AD-U2).

PY - 2006/9

Y1 - 2006/9

N2 - In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin.

AB - In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin.

KW - Avidin

KW - Bio-compatible nanoparticles

KW - Biotinylated alkaline phosphatase

KW - Hybridization

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