Preparation and properties of bio-compatible magnetic Fe 3 O 4 nanoparticles

H. T. Chan, Y. Y. Do, P. L. Huang, P. L. Chien, T. S. Chan, R. S. Liu, C. Y. Huang, Shieh-Yueh Yang, Herng-Er Horng

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe 3 O 4 ) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin.

Original languageEnglish
JournalJournal of Magnetism and Magnetic Materials
Volume304
Issue number1
DOIs
Publication statusPublished - 2006 Sep 1

Fingerprint

biotin
ethylenediamine
Avidin
Biotin
Nanoparticles
nanoparticles
preparation
Ferrosoferric Oxide
Immunosorbents
immunoassay
dextrans
Dextran
Magnetite
Coprecipitation
Dextrans
Surface-Active Agents
magnetite
attachment
enzymes
Assays

Keywords

  • Avidin
  • Bio-compatible nanoparticles
  • Biotinylated alkaline phosphatase
  • Hybridization

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Preparation and properties of bio-compatible magnetic Fe 3 O 4 nanoparticles . / Chan, H. T.; Do, Y. Y.; Huang, P. L.; Chien, P. L.; Chan, T. S.; Liu, R. S.; Huang, C. Y.; Yang, Shieh-Yueh; Horng, Herng-Er.

In: Journal of Magnetism and Magnetic Materials, Vol. 304, No. 1, 01.09.2006.

Research output: Contribution to journalArticle

Chan, H. T. ; Do, Y. Y. ; Huang, P. L. ; Chien, P. L. ; Chan, T. S. ; Liu, R. S. ; Huang, C. Y. ; Yang, Shieh-Yueh ; Horng, Herng-Er. / Preparation and properties of bio-compatible magnetic Fe 3 O 4 nanoparticles In: Journal of Magnetism and Magnetic Materials. 2006 ; Vol. 304, No. 1.
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