Picosecond laser surface modification of aluminum oxide with fish-scale structures for cell culture

Zhao Chi Chen, Tien Li Chang*, Cheng Che Liu, Wen Tse Hsiao, Chih Heng Huang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


The surface modification of a layer substrate has received attention for many biomedical applications (e.g., tissue engineering and biosensing). Under an in situ control, we proposed a strategy for surface modification of hard and brittle ceramics with ultrafast laser pulses for cell culture. A type of ultrafast laser based on the picosecond pulse technique was employed to ablate the surface of a pristine aluminum oxide (Al2O3) substrate. The surface of the formed reproducible micro-nanostructures indicated the fish-scale structures, which was analyzed under different laser ablation conditions. Subsequently, the SV40 T-antigen human embryonic kidney 293 (HEK293T) cells were cultured on the laser-ablated Al2O3 substrates. These microstructures and nanostructures can enhance cell proliferation response. The surface effect played a crucial role, by which the surface roughness (Ra) and contact angle (CA) of water droplets on the ablated Al2O3 substrate increased with the increasing laser fluence. The formed fish-scale structures were formed by the parameters of laser ablation where the structural characteristics of the Al2O3 substrate can be remained in the ultrafast laser process. The presented process provides a simple support for developing ablated structures on the Al2O3 substrate for cell culture.

Original languageEnglish
Pages (from-to)17651-17658
Number of pages8
JournalCeramics International
Issue number11
Publication statusPublished - 2020 Aug 1


  • Aluminum oxide structures
  • Cell culture
  • Fish-scale structures
  • Picosecond laser ablation
  • Surface modification

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Process Chemistry and Technology
  • Surfaces, Coatings and Films
  • Materials Chemistry


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