Phylogeography of Varicorhinus barbatulus (Cyprinidae) in Taiwan based on nucleotide variation of mtDNA and allozymes

Jiang Ping Wang, Hung Du Lin, Shong Huang, Chih Hung Pan, Xiang Lin Chen, Tzen Yuh Chiang

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The phylogeographical patterns and population genetic structures of Varicorhinus barbatulus in Taiwan were investigated based on genetic diversity of 34 allozyme loci and nucleotide sequences of 3′ end of the cytochrome b gene, tRNA genes, D-loop control region, and the 5′ end of the 12S rRNA of mtDNA. Allozyme and mtDNA analyses revealing evident geographical structuring suggest limited gene flow between populations (FST = 0.511 and 0.791, respectively). Low genetic variability within populations (P = 5.56%; He = 0.018) based on allozymes and significantly negative Tajima's D statistics based on mtDNA suggest that most populations in Taiwan may have originated from a small number of founders followed by demographic expansion. The gene genealogy of mtDNA identified six lineages corresponding to major drainages that were separated by the geological barriers due to vicariant events. A minimum spanning network based on nucleotide substitutions reflects divergence from populations of the Miaoli Plateau to northern and southern regions of the island. In contrast to a previous hypothesis that suggests an early invasion to eastern part of Taiwan prior to the lifting of central mountain range some one million years ago, the mtDNA genealogy and molecular dating reveal very recent colonization of the eastern population. Nested clade analyses revealing significant associations between genetic structure and geographical division identify past fragmentation and range expansion as major phylogeographical events that shaped the geographical distribution of this species in Taiwan.

Original languageEnglish
Pages (from-to)1143-1156
Number of pages14
JournalMolecular Phylogenetics and Evolution
Volume31
Issue number3
DOIs
Publication statusPublished - 2004 Jan 1

Fingerprint

Phylogeography
Cyprinidae
phylogeography
allozyme
Mitochondrial DNA
Taiwan
allozymes
Isoenzymes
genealogy
mitochondrial DNA
Nucleotides
nucleotides
genetic structure
gene
Genealogy and Heraldry
Population
Genetic Structures
range expansion
geographical distribution
population genetics

Keywords

  • Allozyme
  • Geographical structuring
  • Nested clade analysis
  • Phylogeography
  • Taiwan
  • Varicorhinus barbatulus
  • mtDNA

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Phylogeography of Varicorhinus barbatulus (Cyprinidae) in Taiwan based on nucleotide variation of mtDNA and allozymes. / Wang, Jiang Ping; Lin, Hung Du; Huang, Shong; Pan, Chih Hung; Chen, Xiang Lin; Chiang, Tzen Yuh.

In: Molecular Phylogenetics and Evolution, Vol. 31, No. 3, 01.01.2004, p. 1143-1156.

Research output: Contribution to journalArticle

Wang, Jiang Ping ; Lin, Hung Du ; Huang, Shong ; Pan, Chih Hung ; Chen, Xiang Lin ; Chiang, Tzen Yuh. / Phylogeography of Varicorhinus barbatulus (Cyprinidae) in Taiwan based on nucleotide variation of mtDNA and allozymes. In: Molecular Phylogenetics and Evolution. 2004 ; Vol. 31, No. 3. pp. 1143-1156.
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