Peroxidase mimicking

Fe(Salen)Cl modified electrodes, fundamental properties and applications for biosensing

Yea Wenn Liou, Chong Mou Wang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Iron(lII) N,N′-bis(salicylidene)ethylenediamine (denoted as Fe(Salen)+) was prepared and characterized for its application in chemical analysis. From the stability constant of Fe(III)(Salen)+ (7.1×1025 M-1) and the formal potentials of Fe3+/2+ and Fe(Salen)+/0, the stability constant of Fe(II)(Salen) was calculated to be 3×1017 M-1. This relatively weaker stability constant, compared with that of Fe(III)(Salen)+, led to the occurrence of the electron transfer reactions between Fe(Salen) and electron acceptors, like oxygen and H2O2. Experimental results supported this hypothesis, showing that the pseudo-first order rate constants for the reactions of Fe(II)(Salen) with O2 (DMSO-H2O, v/v 4:1) and H2O2 (pH 7) are 330 and 4400 M-1 s-1, respectively. Because of this catalytic effect, a sensing electrode for glucose or uric acid was constructed on the basis of Fe(Salen)+ and glucose oxidase (GOx) or uricase (UOx). According to the flow injection analysis (FIA). the detection limits were 1 μm for glucose at pH 7 and 0.1 μM for uric acid at pH 8.5, respectively. The linear response to each substrate covered a region of 1 μM-10 mM for glucose and 5-40 μM for uric acid. Fe(Salen)+ might form a 1:1 adduct with β-cyclodextrin (β-CDx); the equilibrium constant was determined to be about 6 M-1. Although this chemical equilibrium, in terms of the numerical value, was not significant, the formation of {Fe(Salen)}2O was effectively limited as β-CDx was incorporated.

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalJournal of Electroanalytical Chemistry
Volume481
Issue number1
DOIs
Publication statusPublished - 2000 Jan 31

Fingerprint

Peroxidase
Glucose
Electrodes
Acids
Glucose oxidase
Electrons
Cyclodextrins
Equilibrium constants
Uric Acid
Rate constants
ethylenediamine
Iron
Oxygen
Substrates
Chemical analysis
disalicylaldehyde ethylenediamine
Urate Oxidase
Glucose Oxidase
Dimethyl Sulfoxide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

Cite this

Peroxidase mimicking : Fe(Salen)Cl modified electrodes, fundamental properties and applications for biosensing. / Liou, Yea Wenn; Wang, Chong Mou.

In: Journal of Electroanalytical Chemistry, Vol. 481, No. 1, 31.01.2000, p. 102-109.

Research output: Contribution to journalArticle

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abstract = "Iron(lII) N,N′-bis(salicylidene)ethylenediamine (denoted as Fe(Salen)+) was prepared and characterized for its application in chemical analysis. From the stability constant of Fe(III)(Salen)+ (7.1×1025 M-1) and the formal potentials of Fe3+/2+ and Fe(Salen)+/0, the stability constant of Fe(II)(Salen) was calculated to be 3×1017 M-1. This relatively weaker stability constant, compared with that of Fe(III)(Salen)+, led to the occurrence of the electron transfer reactions between Fe(Salen) and electron acceptors, like oxygen and H2O2. Experimental results supported this hypothesis, showing that the pseudo-first order rate constants for the reactions of Fe(II)(Salen) with O2 (DMSO-H2O, v/v 4:1) and H2O2 (pH 7) are 330 and 4400 M-1 s-1, respectively. Because of this catalytic effect, a sensing electrode for glucose or uric acid was constructed on the basis of Fe(Salen)+ and glucose oxidase (GOx) or uricase (UOx). According to the flow injection analysis (FIA). the detection limits were 1 μm for glucose at pH 7 and 0.1 μM for uric acid at pH 8.5, respectively. The linear response to each substrate covered a region of 1 μM-10 mM for glucose and 5-40 μM for uric acid. Fe(Salen)+ might form a 1:1 adduct with β-cyclodextrin (β-CDx); the equilibrium constant was determined to be about 6 M-1. Although this chemical equilibrium, in terms of the numerical value, was not significant, the formation of {Fe(Salen)}2O was effectively limited as β-CDx was incorporated.",
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