Peptide-imprinted conductive polymer on continuous monolayer molybdenum disulfide transferred electrodes for electrochemical sensing of Matrix Metalloproteinase-1 in lung cancer culture medium

Mei Hwa Lee, Cheng Chih Lin, Wlodzimierz Kutner, James L. Thomas, Chu Yun Lin, Zofia Iskierko, Yu Seng Ku, Chien Yu Lin, Pawel Borowicz, Piyush Sindhu Sharma, Yann Wen Lan*, Chien Hsin Yang*, Hung Yin Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Matrix metalloproteinase-1 (MMP-1) is associated with lung cancer, and thus the monitoring of the concentration of this protein may be useful for assessing lung cancer progression. In this work, poly(triphenylamine rhodanine-3-acetic acid-co-3,4-ethoxylene dioxy thiophene)s were incorporated onto a continuous monolayer (cML) of molybdenum disulfide (MoS2) by peptide-imprinted electropolymerization, in which peptides of MMP-1 were used as the templates in epitope imprinting. The morphology of the MoS2 thin film was monitored using optical and scanning electron microscopy. The imprinted MoS2 thin film electrode electrochemically responseded (at 1.0 pg/mL MMP-1) 1.4 times than ordinary imprinted electrodes. Moreover, MoS2 cML coating with PIPs can extend the sensing range of the PIP-coated electrodes from 1.0 pg/mL up to 10.0 pg/mL. The MoS2 cML electrode was used to determine that the MMP-1 concentration in the A549 cell line culture medium was 800 ng/mL. Comparison with results from a commercially available ELISA kit indicated an accuracy of 95 ± 5%.

Original languageEnglish
Article number100258
JournalBiosensors and Bioelectronics: X
Volume13
DOIs
Publication statusPublished - 2023 May

Keywords

  • Electrochemical sensing
  • Matrix Metalloproteinase-1
  • Molecular imprinting
  • Molybdenum disulfide

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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