@article{b743856dcd134b61bafca019d93759ad,
title = "Peptide-imprinted conductive polymer on continuous monolayer molybdenum disulfide transferred electrodes for electrochemical sensing of Matrix Metalloproteinase-1 in lung cancer culture medium",
abstract = "Matrix metalloproteinase-1 (MMP-1) is associated with lung cancer, and thus the monitoring of the concentration of this protein may be useful for assessing lung cancer progression. In this work, poly(triphenylamine rhodanine-3-acetic acid-co-3,4-ethoxylene dioxy thiophene)s were incorporated onto a continuous monolayer (cML) of molybdenum disulfide (MoS2) by peptide-imprinted electropolymerization, in which peptides of MMP-1 were used as the templates in epitope imprinting. The morphology of the MoS2 thin film was monitored using optical and scanning electron microscopy. The imprinted MoS2 thin film electrode electrochemically responseded (at 1.0 pg/mL MMP-1) 1.4 times than ordinary imprinted electrodes. Moreover, MoS2 cML coating with PIPs can extend the sensing range of the PIP-coated electrodes from 1.0 pg/mL up to 10.0 pg/mL. The MoS2 cML electrode was used to determine that the MMP-1 concentration in the A549 cell line culture medium was 800 ng/mL. Comparison with results from a commercially available ELISA kit indicated an accuracy of 95 ± 5%.",
keywords = "Electrochemical sensing, Matrix Metalloproteinase-1, Molecular imprinting, Molybdenum disulfide",
author = "Lee, {Mei Hwa} and Lin, {Cheng Chih} and Wlodzimierz Kutner and Thomas, {James L.} and Lin, {Chu Yun} and Zofia Iskierko and Ku, {Yu Seng} and Lin, {Chien Yu} and Pawel Borowicz and Sharma, {Piyush Sindhu} and Lan, {Yann Wen} and Yang, {Chien Hsin} and Lin, {Hung Yin}",
note = "Funding Information: An acetonitrile (ACN) solution of 1 mM (total) of TPARA and EDOT had 0.5 μg/mL MMP-1 peptide (for imprinting) added to prepare a solution for electropolymerization. (The TPARA was synthesis as previously reported (Yang et al., 2009), and is briefly described in the Supporting Information.) MoS2 cML/ITO electrodes were then immersed to prepare peptide-imprinted (PIPs) MoS2 cML/ITO electrodes (Scheme 1); the same solution, but without MMP-1 peptide, was used to prepare non-imprinted polymer (NIP), control electrodes. The electrodes were connected to a CHI 400C potentiostat (CH Instruments, Inc., Austin TX, USA), and the potential was cycled, at a 0.1 V/s scan rate, between −0.2 and 1.0 V vs. Ag/AgCl. These electrodes were then immersed in a 10 mL of 5 vol% ethanol solution and then rotated at 130 rpm for 5 min on an OSR201-1 orbital shaker (GenePure technology, Taichung, Taiwan), and repeated using DI water. (The same washing procedure was applied to remove the bound peptide or protein for the reusability measurements.)HYL appreciates the financial support of the Ministry of Science and Technology of ROC under Contract Nos. MOST 106-2221-E-390-013-MY3, MOST 107-2923-M-390-001-MY3, MOST 108-2923-B-390-001-MY3, MOST 109-2314-B-390-001-MY3 and MOST 110-2221-E-390-003-MY3. WK acknowledges the National Center for Research and Development (NCBR) of Poland for financial support through Grant No. SensIPF PL-TW/VI/2/2019. Funding Information: HYL appreciates the financial support of the Ministry of Science and Technology of ROC under Contract Nos. MOST 106-2221-E-390-013-MY3 , MOST 107-2923-M-390-001-MY3 , MOST 108-2923-B-390-001-MY3 , MOST 109-2314-B-390-001-MY3 and MOST 110-2221-E-390-003-MY3 . WK acknowledges the National Center for Research and Development ( NCBR ) of Poland for financial support through Grant No. SensIPF PL-TW/VI/2/2019 . Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2023",
month = may,
doi = "10.1016/j.biosx.2022.100258",
language = "English",
volume = "13",
journal = "Biosensors and Bioelectronics: X",
issn = "2590-1370",
publisher = "Elsevier Ltd",
}
