Oral treatment with the herbal formula B401 protects against aging-dependent neurodegeneration by attenuating oxidative stress and apoptosis in the brain of R6/2 mice

Sheue Er Wang, Ching Lung Lin, Chih Hsiang Hsu, Shuenn Jyi Sheu, Chung Hsin Wu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Neurodegeneration is characterized by progressive neurological deficits due to selective neuronal loss in the nervous system. Huntington’s disease (HD) is an incurable neurodegenerative disorder. Neurodegeneration in HD patients shows aging-dependent pattern. Our previous study has suggested that a herbal formula B401 may have neuroprotective effects in the brains of R6/2 mice. Objective: To clarify possible mechanisms for neurodegeneration, which improves the understanding the aging process. This study focuses on clarifying neurodegenerative mechanisms and searching potential therapeutic targets in HD patients. Methods: The oxidative stress and apoptosis were compared in the brain tissue between R6/2 HD mice with and without oral B401 treatment. Expressions of proteins for oxidative stress and apoptosis in the brain tissue of R6/2 HD mice were examined by using immunostaining and Western blotting techniques. Results: R6/2 HD mice with oral B401 treatment significantly reduced reactive oxygen species levels in the blood, but markedly increased expressions of superoxide dismutase 2 in the brain tissue. Furthermore, R6/2 HD mice with oral B401 treatment significantly increased expressions of B-cell lymphoma 2 (Bcl-2), but significantly reduced expressions of Bcl-2-associated X protein (Bax), calpain, and caspase-3 in the brain tissue. Conclusion: Our findings provide evidence that the herbal formula B401 can remedy for aging-dependent neurodegeneration of R6/2 mice via suppressing oxidative stress and apoptosis in the brain. We suggest that the herbal formula B401 can be developed as a potential health supplement for ameliorating aging-dependent neurodegeneration.

Original languageEnglish
Pages (from-to)1825-1837
Number of pages13
JournalClinical Interventions in Aging
Volume10
DOIs
Publication statusPublished - 2015 Nov 6

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Keywords

  • B-cell lymphoma 2
  • Bcl-2-associated X protein
  • Calpain
  • Caspase-3
  • Huntington’s disease
  • Neuroprotection
  • Reactive oxygen species
  • Superoxide dismutase 2
  • Transgenic mouse model

ASJC Scopus subject areas

  • Geriatrics and Gerontology

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