ORA47 (octadecanoid-responsive AP2/ERF-domain transcription factor 47) regulates jasmonic acid and abscisic acid biosynthesis and signaling through binding to a novel cis-element

Hsing Yu Chen, En Jung Hsieh, Mei Chun Cheng, Chien Yu Chen, Shih Ying Hwang, Tsan Piao Lin

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

ORA47 (octadecanoid-responsive AP2/ERF-domain transcription factor 47) of Arabidopsis thaliana is an AP2/ERF domain transcription factor that regulates jasmonate (JA) biosynthesis and is induced by methyl JA treatment. The regulatory mechanism of ORA47 remains unclear. ORA47 is shown to bind to the cis-element (NC/GT)CGNCCA, which is referred to as the O-box, in the promoter of ABI2. We proposed that ORA47 acts as a connection between ABA INSENSITIVE1 (ABI1) and ABI2 and mediates an ABI1-ORA47-ABI2 positive feedback loop. PORA47:ORA47-GFP transgenic plants were used in a chromatin immunoprecipitation (ChIP) assay to show that ORA47 participates in the biosynthesis and/or signaling pathways of nine phytohormones. Specifically, many abscisic acid (ABA) and JA biosynthesis and signaling genes were direct targets of ORA47 under stress conditions. The JA content of the P35S:ORA47-GR lines was highly induced under wounding and moderately induced under water stress relative to that of the wild-type plants. The wounding treatment moderately increased ABA accumulation in the transgenic lines, whereas the water stress treatment repressed the ABA content. ORA47 is proposed to play a role in the biosynthesis of JA and ABA and in regulating the biosynthesis and/or signaling of a suite of phytohormone genes when plants are subjected to wounding and water stress.

Original languageEnglish
Pages (from-to)599-613
Number of pages15
JournalThe New phytologist
Volume211
Issue number2
DOIs
Publication statusPublished - 2016 Jul 1

    Fingerprint

Keywords

  • abscisic acid (ABA)
  • AP2/ERF domain gene
  • Arabidopsis thaliana
  • jasmonic acid (JA)
  • O-box
  • ORA47 (octadecanoid-responsive AP2/ERF-domain transcription factor 47)
  • water stress
  • wounding

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this