TY - JOUR
T1 - Optimization of the separation of malachite green in water by capillary electrophoresis Raman spectroscopy (CE-RS) based on the stacking and sweeping modes
AU - Tsai, Chih Hsin
AU - Lin, Jia Der
AU - Lin, Cheng Huang
N1 - Funding Information:
This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-94-2113-M-003-017.
PY - 2007/4/30
Y1 - 2007/4/30
N2 - A capillary electrophoresis Raman spectroscopy (CE-RS) method based on the stacking and sweeping modes are described. A non-fluorescent compound (malachite green, MG; crystal violet, CV) and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as the model compound and light source, respectively. In order to carry out a quantitative and analysis of MG, a monochromator was used to collect the specific Raman line at 1616 cm-1 (the N-φ and C-C stretching, corresponding to 582 nm when the wavelength of the exciting source is 532 nm). The limit of detection (LOD) for MG was 1.6 × 10-5 and 1.1 × 10-5 M, respectively, based on the CZE and MEKC modes. This could be improved to 3.4 × 10-7 and 5.3 × 10-9 M, respectively, when the stacking and sweeping modes were applied. The method was also extended to the determination of MG in an actual sample.
AB - A capillary electrophoresis Raman spectroscopy (CE-RS) method based on the stacking and sweeping modes are described. A non-fluorescent compound (malachite green, MG; crystal violet, CV) and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as the model compound and light source, respectively. In order to carry out a quantitative and analysis of MG, a monochromator was used to collect the specific Raman line at 1616 cm-1 (the N-φ and C-C stretching, corresponding to 582 nm when the wavelength of the exciting source is 532 nm). The limit of detection (LOD) for MG was 1.6 × 10-5 and 1.1 × 10-5 M, respectively, based on the CZE and MEKC modes. This could be improved to 3.4 × 10-7 and 5.3 × 10-9 M, respectively, when the stacking and sweeping modes were applied. The method was also extended to the determination of MG in an actual sample.
KW - Capillary electrophoresis
KW - Crystal violet
KW - Malachite green
KW - Raman spectroscopy
KW - Stacking
KW - Sweeping
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U2 - 10.1016/j.talanta.2006.10.037
DO - 10.1016/j.talanta.2006.10.037
M3 - Article
AN - SCOPUS:34047271026
SN - 0039-9140
VL - 72
SP - 368
EP - 372
JO - Talanta
JF - Talanta
IS - 2
ER -