Optimization of the separation of malachite green in water by capillary electrophoresis Raman spectroscopy (CE-RS) based on the stacking and sweeping modes

Chih Hsin Tsai; Jia Der Lin; Cheng Huang Lin

doi:10.1016/j.talanta.2006.10.037

Optimization of the separation of malachite green in water by capillary electrophoresis Raman spectroscopy (CE-RS) based on the stacking and sweeping modes

Chih Hsin Tsai, Jia Der Lin, Cheng Huang Lin^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

A capillary electrophoresis Raman spectroscopy (CE-RS) method based on the stacking and sweeping modes are described. A non-fluorescent compound (malachite green, MG; crystal violet, CV) and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as the model compound and light source, respectively. In order to carry out a quantitative and analysis of MG, a monochromator was used to collect the specific Raman line at 1616 cm^-1 (the N-φ and C-C stretching, corresponding to 582 nm when the wavelength of the exciting source is 532 nm). The limit of detection (LOD) for MG was 1.6 × 10^-5 and 1.1 × 10^-5 M, respectively, based on the CZE and MEKC modes. This could be improved to 3.4 × 10^-7 and 5.3 × 10^-9 M, respectively, when the stacking and sweeping modes were applied. The method was also extended to the determination of MG in an actual sample.

Original language	English
Pages (from-to)	368-372
Number of pages	5
Journal	Talanta
Volume	72
Issue number	2
DOIs	https://doi.org/10.1016/j.talanta.2006.10.037
Publication status	Published - 2007 Apr 30

Keywords

Capillary electrophoresis
Crystal violet
Malachite green
Raman spectroscopy
Stacking
Sweeping

ASJC Scopus subject areas

Analytical Chemistry

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10.1016/j.talanta.2006.10.037

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title = "Optimization of the separation of malachite green in water by capillary electrophoresis Raman spectroscopy (CE-RS) based on the stacking and sweeping modes",

abstract = "A capillary electrophoresis Raman spectroscopy (CE-RS) method based on the stacking and sweeping modes are described. A non-fluorescent compound (malachite green, MG; crystal violet, CV) and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as the model compound and light source, respectively. In order to carry out a quantitative and analysis of MG, a monochromator was used to collect the specific Raman line at 1616 cm-1 (the N-φ and C-C stretching, corresponding to 582 nm when the wavelength of the exciting source is 532 nm). The limit of detection (LOD) for MG was 1.6 × 10-5 and 1.1 × 10-5 M, respectively, based on the CZE and MEKC modes. This could be improved to 3.4 × 10-7 and 5.3 × 10-9 M, respectively, when the stacking and sweeping modes were applied. The method was also extended to the determination of MG in an actual sample.",

keywords = "Capillary electrophoresis, Crystal violet, Malachite green, Raman spectroscopy, Stacking, Sweeping",

author = "Tsai, {Chih Hsin} and Lin, {Jia Der} and Lin, {Cheng Huang}",

note = "Funding Information: This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-94-2113-M-003-017.",

year = "2007",

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doi = "10.1016/j.talanta.2006.10.037",

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AU - Tsai, Chih Hsin

AU - Lin, Jia Der

AU - Lin, Cheng Huang

N1 - Funding Information: This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-94-2113-M-003-017.

PY - 2007/4/30

Y1 - 2007/4/30

N2 - A capillary electrophoresis Raman spectroscopy (CE-RS) method based on the stacking and sweeping modes are described. A non-fluorescent compound (malachite green, MG; crystal violet, CV) and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as the model compound and light source, respectively. In order to carry out a quantitative and analysis of MG, a monochromator was used to collect the specific Raman line at 1616 cm-1 (the N-φ and C-C stretching, corresponding to 582 nm when the wavelength of the exciting source is 532 nm). The limit of detection (LOD) for MG was 1.6 × 10-5 and 1.1 × 10-5 M, respectively, based on the CZE and MEKC modes. This could be improved to 3.4 × 10-7 and 5.3 × 10-9 M, respectively, when the stacking and sweeping modes were applied. The method was also extended to the determination of MG in an actual sample.

AB - A capillary electrophoresis Raman spectroscopy (CE-RS) method based on the stacking and sweeping modes are described. A non-fluorescent compound (malachite green, MG; crystal violet, CV) and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as the model compound and light source, respectively. In order to carry out a quantitative and analysis of MG, a monochromator was used to collect the specific Raman line at 1616 cm-1 (the N-φ and C-C stretching, corresponding to 582 nm when the wavelength of the exciting source is 532 nm). The limit of detection (LOD) for MG was 1.6 × 10-5 and 1.1 × 10-5 M, respectively, based on the CZE and MEKC modes. This could be improved to 3.4 × 10-7 and 5.3 × 10-9 M, respectively, when the stacking and sweeping modes were applied. The method was also extended to the determination of MG in an actual sample.

KW - Capillary electrophoresis

KW - Crystal violet

KW - Malachite green

KW - Raman spectroscopy

KW - Stacking

KW - Sweeping

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Optimization of the separation of malachite green in water by capillary electrophoresis Raman spectroscopy (CE-RS) based on the stacking and sweeping modes

Abstract

Keywords

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