Optimization of the separation of lysergic acid diethylamide in urine by a sweeping technique using micellar electrokinetic chromatography

Ching Fang, Ju Tsung Liu, Cheng Huang Lin

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The separation and on-line concentrations of lysergic acid diethylamide (LSD), iso-lysergic acid diethylamide (iso-LSD) and lysergic acid N, N-methylpropylamide (LAMPA) in human urine were investigated by capillary electrophoresis-fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as an anionic surfactant. A number of parameters such as buffer pH, SDS concentration, Brij-30 concentration and the content of organic solvent used in separation, were optimized. The techniques of sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used for determining on-line concentrations. The advantages and disadvantages of this procedure with respect to sensitivity, precision and simplicity are discussed and compared.

Original languageEnglish
Pages (from-to)37-47
Number of pages11
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume775
Issue number1
DOIs
Publication statusPublished - 2002 Jul 25

Fingerprint

Lysergic Acid Diethylamide
Chromatography
Sodium Dodecyl Sulfate
Lysergic Acid
Urine
Capillary electrophoresis
Anionic surfactants
Fluorescence Spectrometry
Fluorescence spectroscopy
Capillary Electrophoresis
Surface-Active Agents
Organic solvents
Cations
Buffers
Injections
Brij 30

Keywords

  • Lysergic acid diethylamide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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