TY - JOUR
T1 - Optimization of separation and online sample concentration of N,N-dimethyltryptamine and related compounds using MEKC
AU - Wang, Man Juing
AU - Tsai, Chih Hsin
AU - Hsu, Wei Ya
AU - Liu, Ju Tsung
AU - Lin, Cheng Huang
PY - 2009/2
Y1 - 2009/2
N2 - The optimal separation conditions and online sample concentration for N,N-dimethyltryptamine (DMT) and related compounds, including α-methyltryptamine (AMT), 5-methoxy-AMT (5-MeO-AMT), N,N-diethyltryptamine (DET), N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT), N,N-diisopropyltryptamine (DiPT), 5-methoxy-DMT (5-MeO-DMT), and 5-methoxy-N,N-DiPT (5-MeO-DiPT), using micellar EKC (MEKC) with UV-absorbance detection are described. The LODs (S/N = 3) for MEKC ranged from 1.0 - 1.8 μg/mL. Use of online sample concentration methods, including sweeping-MEKC and cation-selective exhaustive injection-sweep-MEKC (CSEI-sweep-MEKC) improved the LODs to 2.2 - 8.0 ng/mL and 1.3 - 2.7 ng/mL, respectively. In addition, the order of migration of the nine tryptamines was investigated. A urine sample, obtained by spiking urine collected from a human volunteer with DMT, was also successfully examined.
AB - The optimal separation conditions and online sample concentration for N,N-dimethyltryptamine (DMT) and related compounds, including α-methyltryptamine (AMT), 5-methoxy-AMT (5-MeO-AMT), N,N-diethyltryptamine (DET), N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT), N,N-diisopropyltryptamine (DiPT), 5-methoxy-DMT (5-MeO-DMT), and 5-methoxy-N,N-DiPT (5-MeO-DiPT), using micellar EKC (MEKC) with UV-absorbance detection are described. The LODs (S/N = 3) for MEKC ranged from 1.0 - 1.8 μg/mL. Use of online sample concentration methods, including sweeping-MEKC and cation-selective exhaustive injection-sweep-MEKC (CSEI-sweep-MEKC) improved the LODs to 2.2 - 8.0 ng/mL and 1.3 - 2.7 ng/mL, respectively. In addition, the order of migration of the nine tryptamines was investigated. A urine sample, obtained by spiking urine collected from a human volunteer with DMT, was also successfully examined.
KW - MEKC
KW - Sweeping-MEKC
KW - Tryptamine
UR - http://www.scopus.com/inward/record.url?scp=62749096803&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62749096803&partnerID=8YFLogxK
U2 - 10.1002/jssc.200800492
DO - 10.1002/jssc.200800492
M3 - Article
C2 - 19137528
AN - SCOPUS:62749096803
SN - 1615-9306
VL - 32
SP - 441
EP - 445
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 3
ER -