TY - JOUR
T1 - Optimal production of sulphuric acid by Thiobacillus thiooxidans using response surface methodology
AU - Liu, Hsuan Liang
AU - Lan, Yann Wen
AU - Cheng, Yang Chu
N1 - Funding Information:
Financial support (project numbers: NSC 89-2214-E-027-006 and NSC 90-2214-E-027-006) from the National Science Council, Taiwan, ROC is very much appreciated. The authors also thank Dr. Bor-Yann Chen of I-Lan Institute of Technology for his valuable discussions. The experiments comply with the current laws of Taiwan, ROC in which they were performed.
PY - 2004/10/29
Y1 - 2004/10/29
N2 - Response surface methodology was applied to determine the optimal medium composition for sulphuric acid production by an indigenous Thiobacillus thiooxidans. With a view to reducing the number of experiments and obtaining the mutual interactions between the variables, a 24 full-factorial central composite design was employed for experimental design and analysis of the results. Four independent variables, KH2PO4, (NH 4)2SO4, MgSO4 and elemental sulphur, in the growth medium were tested. Elemental sulphur was found to exhibit positive correlation with the sulphuric acid yield, whereas the other three variables showed no significant effect. No mutual interactions were found between elemental sulphur and each of the other three variables. The optimal composition of the growth medium to achieve the optimal production of sulphuric acid was determined as follows (g/l): KH2PO4=3.5, (NH 4)2SO4=4.9, MgSO4=0.7 and elemental sulphur=23.7. The corresponding sulphuric acid production and dry cell weight were 38 662 ppm and 0.7 g/l, which are about 2.6- and 1.75-fold increase, respectively, compared with those using the conventional T. thiooxidans optimum growth medium.
AB - Response surface methodology was applied to determine the optimal medium composition for sulphuric acid production by an indigenous Thiobacillus thiooxidans. With a view to reducing the number of experiments and obtaining the mutual interactions between the variables, a 24 full-factorial central composite design was employed for experimental design and analysis of the results. Four independent variables, KH2PO4, (NH 4)2SO4, MgSO4 and elemental sulphur, in the growth medium were tested. Elemental sulphur was found to exhibit positive correlation with the sulphuric acid yield, whereas the other three variables showed no significant effect. No mutual interactions were found between elemental sulphur and each of the other three variables. The optimal composition of the growth medium to achieve the optimal production of sulphuric acid was determined as follows (g/l): KH2PO4=3.5, (NH 4)2SO4=4.9, MgSO4=0.7 and elemental sulphur=23.7. The corresponding sulphuric acid production and dry cell weight were 38 662 ppm and 0.7 g/l, which are about 2.6- and 1.75-fold increase, respectively, compared with those using the conventional T. thiooxidans optimum growth medium.
KW - Central composite design
KW - Response surface methodology
KW - Sulphuric acid
KW - Thiobacillus thiooxidans
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U2 - 10.1016/j.procbio.2003.09.018
DO - 10.1016/j.procbio.2003.09.018
M3 - Article
AN - SCOPUS:4344658469
SN - 0032-9592
VL - 39
SP - 1953
EP - 1961
JO - Process Biochemistry
JF - Process Biochemistry
IS - 12
ER -