Optimal production of sulphuric acid by Thiobacillus thiooxidans using response surface methodology

Hsuan Liang Liu, Yann Wen Lan, Yang Chu Cheng

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107 Citations (Scopus)

Abstract

Response surface methodology was applied to determine the optimal medium composition for sulphuric acid production by an indigenous Thiobacillus thiooxidans. With a view to reducing the number of experiments and obtaining the mutual interactions between the variables, a 24 full-factorial central composite design was employed for experimental design and analysis of the results. Four independent variables, KH2PO4, (NH 4)2SO4, MgSO4 and elemental sulphur, in the growth medium were tested. Elemental sulphur was found to exhibit positive correlation with the sulphuric acid yield, whereas the other three variables showed no significant effect. No mutual interactions were found between elemental sulphur and each of the other three variables. The optimal composition of the growth medium to achieve the optimal production of sulphuric acid was determined as follows (g/l): KH2PO4=3.5, (NH 4)2SO4=4.9, MgSO4=0.7 and elemental sulphur=23.7. The corresponding sulphuric acid production and dry cell weight were 38 662 ppm and 0.7 g/l, which are about 2.6- and 1.75-fold increase, respectively, compared with those using the conventional T. thiooxidans optimum growth medium.

Original languageEnglish
Pages (from-to)1953-1961
Number of pages9
JournalProcess Biochemistry
Volume39
Issue number12
DOIs
Publication statusPublished - 2004 Oct 29
Externally publishedYes

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Keywords

  • Central composite design
  • Response surface methodology
  • Sulphuric acid
  • Thiobacillus thiooxidans

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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