Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Yu Chih Lin; Chau Jern Cheng; Ting Chung Poon

doi:10.1364/AO.50.000B25

Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Yu Chih Lin
, Chau Jern Cheng^*
, Ting Chung Poon

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.

Original language	English
Pages (from-to)	B25-B30
Journal	Applied Optics
Volume	50
Issue number	7
DOIs	https://doi.org/10.1364/AO.50.000B25
Publication status	Published - 2011 Mar 1

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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abstract = "The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.",

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AB - The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.

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Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Abstract

ASJC Scopus subject areas

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