Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Yu Chih Lin; Chau Jern Cheng; Ting Chung Poon

doi:10.1364/AO.50.000B25

Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Yu Chih Lin, Chau Jern Cheng^*, Ting Chung Poon

^*Corresponding author for this work

Undergraduate Program of Electro-Optical Engineering

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.

Original language	English
Pages (from-to)	B25-B30
Journal	Applied Optics
Volume	50
Issue number	7
DOIs	https://doi.org/10.1364/AO.50.000B25
Publication status	Published - 2011 Mar 1

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

Access to Document

10.1364/AO.50.000B25

Cite this

@article{d27c57beb57e4d16a1f8046677205ee3,

title = "Optical sectioning with a low-coherence phase-shifting digital holographic microscope",

abstract = "The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.",

author = "Lin, {Yu Chih} and Cheng, {Chau Jern} and Poon, {Ting Chung}",

year = "2011",

month = mar,

day = "1",

doi = "10.1364/AO.50.000B25",

language = "English",

volume = "50",

pages = "B25--B30",

journal = "Applied Optics",

issn = "1559-128X",

publisher = "The Optical Society",

number = "7",

}

TY - JOUR

T1 - Optical sectioning with a low-coherence phase-shifting digital holographic microscope

AU - Lin, Yu Chih

AU - Cheng, Chau Jern

AU - Poon, Ting Chung

PY - 2011/3/1

Y1 - 2011/3/1

N2 - The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.

AB - The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.

UR - http://www.scopus.com/inward/record.url?scp=79952170055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952170055&partnerID=8YFLogxK

U2 - 10.1364/AO.50.000B25

DO - 10.1364/AO.50.000B25

M3 - Article

AN - SCOPUS:79952170055

SN - 1559-128X

VL - 50

SP - B25-B30

JO - Applied Optics

JF - Applied Optics

IS - 7

ER -

Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this