Abstract
The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.
Original language | English |
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Pages (from-to) | B25-B30 |
Journal | Applied Optics |
Volume | 50 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2011 Mar 1 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Engineering (miscellaneous)
- Electrical and Electronic Engineering