Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Yu Chih Lin, Chau Jern Cheng*, Ting Chung Poon

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    37 Citations (Scopus)

    Abstract

    The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.

    Original languageEnglish
    Pages (from-to)B25-B30
    JournalApplied Optics
    Volume50
    Issue number7
    DOIs
    Publication statusPublished - 2011 Mar 1

    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics
    • Engineering (miscellaneous)
    • Electrical and Electronic Engineering

    Fingerprint

    Dive into the research topics of 'Optical sectioning with a low-coherence phase-shifting digital holographic microscope'. Together they form a unique fingerprint.

    Cite this