Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Yu Chih Lin, Chau Jern Cheng, Ting Chung Poon

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.

Original languageEnglish
Pages (from-to)B25-B30
JournalApplied Optics
Volume50
Issue number7
DOIs
Publication statusPublished - 2011 Mar 1

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phase coherence
Microscopes
microscopes
Fish
spine
Visualization
fishes
Membranes
Imaging techniques
membranes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)

Cite this

Optical sectioning with a low-coherence phase-shifting digital holographic microscope. / Lin, Yu Chih; Cheng, Chau Jern; Poon, Ting Chung.

In: Applied Optics, Vol. 50, No. 7, 01.03.2011, p. B25-B30.

Research output: Contribution to journalArticle

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