Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Yu Chih Lin, Chau Jern Cheng, Ting Chung Poon

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33 Citations (Scopus)


The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3μm and axial resolution of 10μm through a thickness of 300μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.

Original languageEnglish
Pages (from-to)B25-B30
JournalApplied Optics
Issue number7
Publication statusPublished - 2011 Mar 1


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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