Abstract
Transcriptional factors (TF) are proteins that bind to specific bases of DNA using DNA binding domains to carry out the process of transcription. Identification of TF binding sites is essential for understanding the regulatory circuits that control cellular processes such as cell division and differentiation as well as metabolic and physiological balance [1]. In this work, we deploy a proof-of-concept method adopting bio-conjugation, nanofluidic devices and single molecule techniques for direct mapping of TF binding sites on field stretched single DNA molecules. Here we demonstrated a simple and robust system for TF binding site identification, complementary to the existing techniques practiced in this field.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1873-1875 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| Publication status | Published - 2012 Jan 1 |
| Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 2012 Oct 28 → 2012 Nov 1 |
Other
| Other | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|---|
| Country | Japan |
| City | Okinawa |
| Period | 12/10/28 → 12/11/1 |
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Keywords
- Fluorescence imaging
- Microfluidics
- Nanofluidics
- Single molecule studies
- Transcriptional factors
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices. / Sriram, K. K.; Yeh, Jia Wei; Lin, Yii Lih; Chang, Yi-Ren; Chou, Chia Fu.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1873-1875.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices
AU - Sriram, K. K.
AU - Yeh, Jia Wei
AU - Lin, Yii Lih
AU - Chang, Yi-Ren
AU - Chou, Chia Fu
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Transcriptional factors (TF) are proteins that bind to specific bases of DNA using DNA binding domains to carry out the process of transcription. Identification of TF binding sites is essential for understanding the regulatory circuits that control cellular processes such as cell division and differentiation as well as metabolic and physiological balance [1]. In this work, we deploy a proof-of-concept method adopting bio-conjugation, nanofluidic devices and single molecule techniques for direct mapping of TF binding sites on field stretched single DNA molecules. Here we demonstrated a simple and robust system for TF binding site identification, complementary to the existing techniques practiced in this field.
AB - Transcriptional factors (TF) are proteins that bind to specific bases of DNA using DNA binding domains to carry out the process of transcription. Identification of TF binding sites is essential for understanding the regulatory circuits that control cellular processes such as cell division and differentiation as well as metabolic and physiological balance [1]. In this work, we deploy a proof-of-concept method adopting bio-conjugation, nanofluidic devices and single molecule techniques for direct mapping of TF binding sites on field stretched single DNA molecules. Here we demonstrated a simple and robust system for TF binding site identification, complementary to the existing techniques practiced in this field.
KW - Fluorescence imaging
KW - Microfluidics
KW - Nanofluidics
KW - Single molecule studies
KW - Transcriptional factors
UR - http://www.scopus.com/inward/record.url?scp=84901748095&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901748095&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901748095
SN - 9780979806452
SP - 1873
EP - 1875
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
ER -