Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices

K. K. Sriram, Jia Wei Yeh, Yii Lih Lin, Yi-Ren Chang, Chia Fu Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Transcriptional factors (TF) are proteins that bind to specific bases of DNA using DNA binding domains to carry out the process of transcription. Identification of TF binding sites is essential for understanding the regulatory circuits that control cellular processes such as cell division and differentiation as well as metabolic and physiological balance [1]. In this work, we deploy a proof-of-concept method adopting bio-conjugation, nanofluidic devices and single molecule techniques for direct mapping of TF binding sites on field stretched single DNA molecules. Here we demonstrated a simple and robust system for TF binding site identification, complementary to the existing techniques practiced in this field.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1873-1875
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • Fluorescence imaging
  • Microfluidics
  • Nanofluidics
  • Single molecule studies
  • Transcriptional factors

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Sriram, K. K., Yeh, J. W., Lin, Y. L., Chang, Y-R., & Chou, C. F. (2012). Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1873-1875). Chemical and Biological Microsystems Society.