Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices

K. K. Sriram; Jia Wei Yeh; Yii Lih Lin; Yi-Ren Chang; Chia Fu Chou

Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices

K. K. Sriram, Jia Wei Yeh, Yii Lih Lin, Yi-Ren Chang, Chia Fu Chou

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Transcriptional factors (TF) are proteins that bind to specific bases of DNA using DNA binding domains to carry out the process of transcription. Identification of TF binding sites is essential for understanding the regulatory circuits that control cellular processes such as cell division and differentiation as well as metabolic and physiological balance [1]. In this work, we deploy a proof-of-concept method adopting bio-conjugation, nanofluidic devices and single molecule techniques for direct mapping of TF binding sites on field stretched single DNA molecules. Here we demonstrated a simple and robust system for TF binding site identification, complementary to the existing techniques practiced in this field.

Original language	English
Title of host publication	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publisher	Chemical and Biological Microsystems Society
Pages	1873-1875
Number of pages	3
ISBN (Print)	9780979806452
Publication status	Published - 2012 Jan 1
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 2012 Oct 28 → 2012 Nov 1

Other

Other	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country	Japan
City	Okinawa
Period	2012/10/28 → 2012/11/01

Keywords

Fluorescence imaging
Microfluidics
Nanofluidics
Single molecule studies
Transcriptional factors

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

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Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices. / Sriram, K. K.; Yeh, Jia Wei; Lin, Yii Lih; Chang, Yi-Ren; Chou, Chia Fu.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1873-1875.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sriram, KK, Yeh, JW, Lin, YL, Chang, Y-R & Chou, CF 2012, Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1873-1875, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 2012/10/28.

Sriram, K. K. ; Yeh, Jia Wei ; Lin, Yii Lih ; Chang, Yi-Ren ; Chou, Chia Fu. / Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1873-1875

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AU - Yeh, Jia Wei

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AU - Chou, Chia Fu

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AB - Transcriptional factors (TF) are proteins that bind to specific bases of DNA using DNA binding domains to carry out the process of transcription. Identification of TF binding sites is essential for understanding the regulatory circuits that control cellular processes such as cell division and differentiation as well as metabolic and physiological balance [1]. In this work, we deploy a proof-of-concept method adopting bio-conjugation, nanofluidic devices and single molecule techniques for direct mapping of TF binding sites on field stretched single DNA molecules. Here we demonstrated a simple and robust system for TF binding site identification, complementary to the existing techniques practiced in this field.

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KW - Microfluidics

KW - Nanofluidics

KW - Single molecule studies

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Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices

Abstract

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Keywords

ASJC Scopus subject areas

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