On-line identification of trans- and cis-resveratrol by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K

Cheng Huang Lin, Yi Hsiu Chen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

This work presents a novel method for the accurate determining trans- and cis-resveratrol (3,5,4′-trihydroxystilbene) by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K. The proposed method permits not only the separation of resveratrol isomers, but also ensures that on-line spectra are readily distinguishable and unambiguously assigned. The experimental results also indicate that the effect of nonaqueous capillary electrophoresis buffer and low-temperature technique increase the detection limit by more than 150-fold.

Original languageEnglish
Pages (from-to)2574-2579
Number of pages6
JournalElectrophoresis
Volume22
Issue number12
DOIs
Publication statusPublished - 2001 Aug 20

Fingerprint

Capillary electrophoresis
Fluorescence Spectrometry
Fluorescence spectroscopy
Capillary Electrophoresis
Isomers
Limit of Detection
Buffers
Temperature
resveratrol

Keywords

  • 77 K fluorescence spectroscopy
  • Nonaqueous capillary electrophoresis
  • Trans- and cis-resveratrol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

On-line identification of trans- and cis-resveratrol by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K. / Lin, Cheng Huang; Chen, Yi Hsiu.

In: Electrophoresis, Vol. 22, No. 12, 20.08.2001, p. 2574-2579.

Research output: Contribution to journalArticle

@article{df1cce4838184dea918a1037520f079f,
title = "On-line identification of trans- and cis-resveratrol by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K",
abstract = "This work presents a novel method for the accurate determining trans- and cis-resveratrol (3,5,4′-trihydroxystilbene) by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K. The proposed method permits not only the separation of resveratrol isomers, but also ensures that on-line spectra are readily distinguishable and unambiguously assigned. The experimental results also indicate that the effect of nonaqueous capillary electrophoresis buffer and low-temperature technique increase the detection limit by more than 150-fold.",
keywords = "77 K fluorescence spectroscopy, Nonaqueous capillary electrophoresis, Trans- and cis-resveratrol",
author = "Lin, {Cheng Huang} and Chen, {Yi Hsiu}",
year = "2001",
month = "8",
day = "20",
doi = "10.1002/1522-2683(200107)22:12<2574::AID-ELPS2574>3.0.CO;2-M",
language = "English",
volume = "22",
pages = "2574--2579",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "12",

}

TY - JOUR

T1 - On-line identification of trans- and cis-resveratrol by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K

AU - Lin, Cheng Huang

AU - Chen, Yi Hsiu

PY - 2001/8/20

Y1 - 2001/8/20

N2 - This work presents a novel method for the accurate determining trans- and cis-resveratrol (3,5,4′-trihydroxystilbene) by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K. The proposed method permits not only the separation of resveratrol isomers, but also ensures that on-line spectra are readily distinguishable and unambiguously assigned. The experimental results also indicate that the effect of nonaqueous capillary electrophoresis buffer and low-temperature technique increase the detection limit by more than 150-fold.

AB - This work presents a novel method for the accurate determining trans- and cis-resveratrol (3,5,4′-trihydroxystilbene) by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K. The proposed method permits not only the separation of resveratrol isomers, but also ensures that on-line spectra are readily distinguishable and unambiguously assigned. The experimental results also indicate that the effect of nonaqueous capillary electrophoresis buffer and low-temperature technique increase the detection limit by more than 150-fold.

KW - 77 K fluorescence spectroscopy

KW - Nonaqueous capillary electrophoresis

KW - Trans- and cis-resveratrol

UR - http://www.scopus.com/inward/record.url?scp=0034898762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034898762&partnerID=8YFLogxK

U2 - 10.1002/1522-2683(200107)22:12<2574::AID-ELPS2574>3.0.CO;2-M

DO - 10.1002/1522-2683(200107)22:12<2574::AID-ELPS2574>3.0.CO;2-M

M3 - Article

C2 - 11519961

AN - SCOPUS:0034898762

VL - 22

SP - 2574

EP - 2579

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 12

ER -