Novel water-soluble hexa(sulfobutyl)fullerene attenuates apoptosis formation after ischemic renal failure

Chiang Ting Chien; Chau Fong Chen; Long Y. Chiang; Ming Kuen Lai

doi:10.1080/10641229909351359

Novel water-soluble hexa(sulfobutyl)fullerene attenuates apoptosis formation after ischemic renal failure

Chiang Ting Chien
, Chau Fong Chen
, Long Y. Chiang
, Ming Kuen Lai

Research output: Contribution to journal › Conference article › peer-review

8 Citations (Scopus)

Abstract

The mechanism for apoptosis induction during the implantation of renal transplants or ischemia/reperfusion (I/R) injury might be related to the release of free radicals. Hexa(sulfobutyl)fullerene, a strong free radical scavenger, is an isomeric mixture of polyfunctional C₆₀ derivative carrying six sulfobutyl groups. To elucidate the role of hexa(sulfobutyl)fullerene in the I/R induced renal damage of the rat kidneys, we used TUNEL, DNA laddering, quantitative PCR and western blot to evaluate the expression of apoptosis. We found that less apoptotic expression and up-regulation in bcl-x_L, an anti-death gene, was found in the damaged kidneys with hexa(sulfobutyl)fullerene (100 μ g) pretreatment. It is suggested that the mechanism for hexa(sulfobutyl)fullerene attenuating I/R induced apoptosis in the rat kidney may be ascribed to the overexpression of bcl-x_L.

Original language	English
Pages (from-to)	529-540
Number of pages	12
Journal	Fullerene Science and Technology
Volume	7
Issue number	4
DOIs	https://doi.org/10.1080/10641229909351359
Publication status	Published - 1999
Externally published	Yes

ASJC Scopus subject areas

General Chemical Engineering

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10.1080/10641229909351359

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@article{3c90c65f37d34c3ab22240ec77249e66,

title = "Novel water-soluble hexa(sulfobutyl)fullerene attenuates apoptosis formation after ischemic renal failure",

abstract = "The mechanism for apoptosis induction during the implantation of renal transplants or ischemia/reperfusion (I/R) injury might be related to the release of free radicals. Hexa(sulfobutyl)fullerene, a strong free radical scavenger, is an isomeric mixture of polyfunctional C60 derivative carrying six sulfobutyl groups. To elucidate the role of hexa(sulfobutyl)fullerene in the I/R induced renal damage of the rat kidneys, we used TUNEL, DNA laddering, quantitative PCR and western blot to evaluate the expression of apoptosis. We found that less apoptotic expression and up-regulation in bcl-xL, an anti-death gene, was found in the damaged kidneys with hexa(sulfobutyl)fullerene (100 μ g) pretreatment. It is suggested that the mechanism for hexa(sulfobutyl)fullerene attenuating I/R induced apoptosis in the rat kidney may be ascribed to the overexpression of bcl-xL.",

author = "Chien, \{Chiang Ting\} and Chen, \{Chau Fong\} and Chiang, \{Long Y.\} and Lai, \{Ming Kuen\}",

note = "Funding Information: Acknowledgment This work was supported by the National Science Council",

year = "1999",

doi = "10.1080/10641229909351359",

language = "English",

volume = "7",

pages = "529--540",

journal = "Fullerene Science and Technology",

issn = "1064-122X",

publisher = "Marcel Dekker Inc.",

number = "4",

}

TY - JOUR

T1 - Novel water-soluble hexa(sulfobutyl)fullerene attenuates apoptosis formation after ischemic renal failure

AU - Chien, Chiang Ting

AU - Chen, Chau Fong

AU - Chiang, Long Y.

AU - Lai, Ming Kuen

N1 - Funding Information: Acknowledgment This work was supported by the National Science Council

PY - 1999

Y1 - 1999

N2 - The mechanism for apoptosis induction during the implantation of renal transplants or ischemia/reperfusion (I/R) injury might be related to the release of free radicals. Hexa(sulfobutyl)fullerene, a strong free radical scavenger, is an isomeric mixture of polyfunctional C60 derivative carrying six sulfobutyl groups. To elucidate the role of hexa(sulfobutyl)fullerene in the I/R induced renal damage of the rat kidneys, we used TUNEL, DNA laddering, quantitative PCR and western blot to evaluate the expression of apoptosis. We found that less apoptotic expression and up-regulation in bcl-xL, an anti-death gene, was found in the damaged kidneys with hexa(sulfobutyl)fullerene (100 μ g) pretreatment. It is suggested that the mechanism for hexa(sulfobutyl)fullerene attenuating I/R induced apoptosis in the rat kidney may be ascribed to the overexpression of bcl-xL.

AB - The mechanism for apoptosis induction during the implantation of renal transplants or ischemia/reperfusion (I/R) injury might be related to the release of free radicals. Hexa(sulfobutyl)fullerene, a strong free radical scavenger, is an isomeric mixture of polyfunctional C60 derivative carrying six sulfobutyl groups. To elucidate the role of hexa(sulfobutyl)fullerene in the I/R induced renal damage of the rat kidneys, we used TUNEL, DNA laddering, quantitative PCR and western blot to evaluate the expression of apoptosis. We found that less apoptotic expression and up-regulation in bcl-xL, an anti-death gene, was found in the damaged kidneys with hexa(sulfobutyl)fullerene (100 μ g) pretreatment. It is suggested that the mechanism for hexa(sulfobutyl)fullerene attenuating I/R induced apoptosis in the rat kidney may be ascribed to the overexpression of bcl-xL.

UR - https://www.scopus.com/pages/publications/0032599620

UR - https://www.scopus.com/pages/publications/0032599620#tab=citedBy

U2 - 10.1080/10641229909351359

DO - 10.1080/10641229909351359

M3 - Conference article

AN - SCOPUS:0032599620

SN - 1064-122X

VL - 7

SP - 529

EP - 540

JO - Fullerene Science and Technology

JF - Fullerene Science and Technology

IS - 4

ER -

Novel water-soluble hexa(sulfobutyl)fullerene attenuates apoptosis formation after ischemic renal failure

Abstract

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