TY - JOUR
T1 - Novel water-soluble hexa(sulfobutyl)fullerene attenuates apoptosis formation after ischemic renal failure
AU - Chien, Chiang Ting
AU - Chen, Chau Fong
AU - Chiang, Long Y.
AU - Lai, Ming Kuen
N1 - Funding Information:
Acknowledgment This work was supported by the National Science Council
PY - 1999
Y1 - 1999
N2 - The mechanism for apoptosis induction during the implantation of renal transplants or ischemia/reperfusion (I/R) injury might be related to the release of free radicals. Hexa(sulfobutyl)fullerene, a strong free radical scavenger, is an isomeric mixture of polyfunctional C60 derivative carrying six sulfobutyl groups. To elucidate the role of hexa(sulfobutyl)fullerene in the I/R induced renal damage of the rat kidneys, we used TUNEL, DNA laddering, quantitative PCR and western blot to evaluate the expression of apoptosis. We found that less apoptotic expression and up-regulation in bcl-xL, an anti-death gene, was found in the damaged kidneys with hexa(sulfobutyl)fullerene (100 μ g) pretreatment. It is suggested that the mechanism for hexa(sulfobutyl)fullerene attenuating I/R induced apoptosis in the rat kidney may be ascribed to the overexpression of bcl-xL.
AB - The mechanism for apoptosis induction during the implantation of renal transplants or ischemia/reperfusion (I/R) injury might be related to the release of free radicals. Hexa(sulfobutyl)fullerene, a strong free radical scavenger, is an isomeric mixture of polyfunctional C60 derivative carrying six sulfobutyl groups. To elucidate the role of hexa(sulfobutyl)fullerene in the I/R induced renal damage of the rat kidneys, we used TUNEL, DNA laddering, quantitative PCR and western blot to evaluate the expression of apoptosis. We found that less apoptotic expression and up-regulation in bcl-xL, an anti-death gene, was found in the damaged kidneys with hexa(sulfobutyl)fullerene (100 μ g) pretreatment. It is suggested that the mechanism for hexa(sulfobutyl)fullerene attenuating I/R induced apoptosis in the rat kidney may be ascribed to the overexpression of bcl-xL.
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U2 - 10.1080/10641229909351359
DO - 10.1080/10641229909351359
M3 - Conference article
AN - SCOPUS:0032599620
SN - 1064-122X
VL - 7
SP - 529
EP - 540
JO - Fullerene Science and Technology
JF - Fullerene Science and Technology
IS - 4
ER -