Novel trypsin-FITC@MOF bioreactor efficiently catalyzes protein digestion

Wan Ling Liu; Sheng Han Lo; Brenda Singco; Chun Chuen Yang; Hsi Ya Huang; Chia Her Lin

doi:10.1039/c3tb00257h

Novel trypsin-FITC@MOF bioreactor efficiently catalyzes protein digestion

Wan Ling Liu, Sheng Han Lo, Brenda Singco, Chun Chuen Yang, Hsi Ya Huang^*, Chia Her Lin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

162 Citations (Scopus)

Abstract

A simple nanoporous metal organic framework (MOF) bioreactor preparation via a low energy 30 minute vortex as driving force to immobilize trypsin enzyme is presented. No chemical modifications were employed thus organic wastes were eliminated. The digestion efficiency of this trypsin bioreactor is exceptionally high, even when reused. This "green" synthesis procedure produced a "green" bioreactor.

Original language	English
Pages (from-to)	928-932
Number of pages	5
Journal	Journal of Materials Chemistry B
Volume	1
Issue number	7
DOIs	https://doi.org/10.1039/c3tb00257h
Publication status	Published - 2013 Feb 21
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
Biomedical Engineering
General Materials Science

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10.1039/c3tb00257h

Cite this

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AU - Lo, Sheng Han

AU - Singco, Brenda

AU - Yang, Chun Chuen

AU - Huang, Hsi Ya

AU - Lin, Chia Her

PY - 2013/2/21

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AB - A simple nanoporous metal organic framework (MOF) bioreactor preparation via a low energy 30 minute vortex as driving force to immobilize trypsin enzyme is presented. No chemical modifications were employed thus organic wastes were eliminated. The digestion efficiency of this trypsin bioreactor is exceptionally high, even when reused. This "green" synthesis procedure produced a "green" bioreactor.

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Novel trypsin-FITC@MOF bioreactor efficiently catalyzes protein digestion

Abstract

ASJC Scopus subject areas

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