Abstract
Highly optically active nonlinear bio-photonic crystalline and semicrystalline structures in living cells were studied by a novel multimodal nonlinear microscopy. Numerous biological structures, including stacked membranes and aligned protein structures are highly organized on a nanoscale and have been found to exhibit strong optical activities through second-harmonic generation (SHG) interactions, behaving similarly to man-made nonlinear photonic crystals. The microscopic technology used in this study is based on a combination of different imaging modes including SHG, third-harmonic generation, and multiphoton-induced fluorescence. With no energy release during harmonic generation processes, the nonlinear-photonic-crystal-like SHG activity is useful for investigating the dynamics of structure-function relationships at subcellular levels and is ideal for studying living cells, as minimal or no preparation is required.
Original language | English |
---|---|
Pages (from-to) | 190-200 |
Number of pages | 11 |
Journal | Journal of Microscopy |
Volume | 208 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2002 Dec |
Keywords
- Cr: forsterite laser
- Multiphoton process
- Nonlinear microscopy
- Photonic crystal
- Second-harmonic generation
- Third harmonic generation
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology