NMR assignments of the WBSCR27 protein related to Williams-Beuren syndrome

Sofia S. Mariasina; Olga A. Petrova; Ilya A. Osterman; Olga V. Sergeeva; Sergey V. Efimov; Vladimir V. Klochkov; Petr V. Sergiev; Olga A. Dontsova; Tai huang Huang; Chi Fon Chang; Vladimir I. Polshakov

doi:10.1007/s12104-018-9827-2

NMR assignments of the WBSCR27 protein related to Williams-Beuren syndrome

Sofia S. Mariasina, Olga A. Petrova, Ilya A. Osterman, Olga V. Sergeeva, Sergey V. Efimov, Vladimir V. Klochkov, Petr V. Sergiev, Olga A. Dontsova, Tai huang Huang, Chi Fon Chang, Vladimir I. Polshakov^*

^*Corresponding author for this work

Department of Physics

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Williams-Beuren syndrome is a genetic disorder characterized by physiological and mental abnormalities, and is caused by hemizygous deletion of several genes in chromosome 7. One of the removed genes encodes the WBSCR27 protein. Bioinformatic analysis of the sequence of WBSCR27 indicates that it belongs to the family of SAM-dependent methyltransferases. However, exact cellular functions of this protein or phenotypic consequences of its deficiency are still unknown. Here we report nearly complete ¹H, ¹⁵N, and ¹³C chemical shifts assignments of the 26 kDa WBSCR27 protein from Mus musculus in complex with the cofactor S-adenosyl-l-methionine (SAM). Analysis of the assigned chemical shifts allowed us to characterize the protein’s secondary structure and backbone dynamics. The topology of the protein’s fold confirms the assumption that the WBSCR27 protein belongs to the family of class I methyltransferases.

Original language	English
Pages (from-to)	303-308
Number of pages	6
Journal	Biomolecular NMR Assignments
Volume	12
Issue number	2
DOIs	https://doi.org/10.1007/s12104-018-9827-2
Publication status	Published - 2018 Oct 1

Keywords

Protein NMR
Resonance assignment
SAM-dependent methyltransferases
Secondary structure
Williams-Beuren syndrome

ASJC Scopus subject areas

Structural Biology
Biochemistry

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10.1007/s12104-018-9827-2

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title = "NMR assignments of the WBSCR27 protein related to Williams-Beuren syndrome",

abstract = "Williams-Beuren syndrome is a genetic disorder characterized by physiological and mental abnormalities, and is caused by hemizygous deletion of several genes in chromosome 7. One of the removed genes encodes the WBSCR27 protein. Bioinformatic analysis of the sequence of WBSCR27 indicates that it belongs to the family of SAM-dependent methyltransferases. However, exact cellular functions of this protein or phenotypic consequences of its deficiency are still unknown. Here we report nearly complete 1H, 15N, and 13C chemical shifts assignments of the 26 kDa WBSCR27 protein from Mus musculus in complex with the cofactor S-adenosyl-l-methionine (SAM). Analysis of the assigned chemical shifts allowed us to characterize the protein{\textquoteright}s secondary structure and backbone dynamics. The topology of the protein{\textquoteright}s fold confirms the assumption that the WBSCR27 protein belongs to the family of class I methyltransferases.",

keywords = "Protein NMR, Resonance assignment, SAM-dependent methyltransferases, Secondary structure, Williams-Beuren syndrome",

author = "Mariasina, {Sofia S.} and Petrova, {Olga A.} and Osterman, {Ilya A.} and Sergeeva, {Olga V.} and Efimov, {Sergey V.} and Klochkov, {Vladimir V.} and Sergiev, {Petr V.} and Dontsova, {Olga A.} and Huang, {Tai huang} and Chang, {Chi Fon} and Polshakov, {Vladimir I.}",

note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media B.V., part of Springer Nature.",

year = "2018",

month = oct,

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doi = "10.1007/s12104-018-9827-2",

language = "English",

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T1 - NMR assignments of the WBSCR27 protein related to Williams-Beuren syndrome

AU - Mariasina, Sofia S.

AU - Petrova, Olga A.

AU - Osterman, Ilya A.

AU - Sergeeva, Olga V.

AU - Efimov, Sergey V.

AU - Klochkov, Vladimir V.

AU - Sergiev, Petr V.

AU - Dontsova, Olga A.

AU - Huang, Tai huang

AU - Chang, Chi Fon

AU - Polshakov, Vladimir I.

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Williams-Beuren syndrome is a genetic disorder characterized by physiological and mental abnormalities, and is caused by hemizygous deletion of several genes in chromosome 7. One of the removed genes encodes the WBSCR27 protein. Bioinformatic analysis of the sequence of WBSCR27 indicates that it belongs to the family of SAM-dependent methyltransferases. However, exact cellular functions of this protein or phenotypic consequences of its deficiency are still unknown. Here we report nearly complete 1H, 15N, and 13C chemical shifts assignments of the 26 kDa WBSCR27 protein from Mus musculus in complex with the cofactor S-adenosyl-l-methionine (SAM). Analysis of the assigned chemical shifts allowed us to characterize the protein’s secondary structure and backbone dynamics. The topology of the protein’s fold confirms the assumption that the WBSCR27 protein belongs to the family of class I methyltransferases.

AB - Williams-Beuren syndrome is a genetic disorder characterized by physiological and mental abnormalities, and is caused by hemizygous deletion of several genes in chromosome 7. One of the removed genes encodes the WBSCR27 protein. Bioinformatic analysis of the sequence of WBSCR27 indicates that it belongs to the family of SAM-dependent methyltransferases. However, exact cellular functions of this protein or phenotypic consequences of its deficiency are still unknown. Here we report nearly complete 1H, 15N, and 13C chemical shifts assignments of the 26 kDa WBSCR27 protein from Mus musculus in complex with the cofactor S-adenosyl-l-methionine (SAM). Analysis of the assigned chemical shifts allowed us to characterize the protein’s secondary structure and backbone dynamics. The topology of the protein’s fold confirms the assumption that the WBSCR27 protein belongs to the family of class I methyltransferases.

KW - Protein NMR

KW - Resonance assignment

KW - SAM-dependent methyltransferases

KW - Secondary structure

KW - Williams-Beuren syndrome

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NMR assignments of the WBSCR27 protein related to Williams-Beuren syndrome

Abstract

Keywords

ASJC Scopus subject areas

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