Neuroprotective Effects of the Herbal Formula B401 in Both Cell and Mouse Models of Alzheimer's Disease

Chih Hsiang Hsu, Sheue Er Wang, Ching Lung Lin, Chun Jen Hsiao, Shuenn Jyi Sheu, Chung Hsin Wu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, we have reported the herbal formula B401 that has neuroprotective effects via multifunction, multitarget characteristics. It is possible that the herbal formula B401 may also provide new insights for AD. Here, we studied protective effects in the Tet-On Aβ 42-GFP SH-SY5Y cell model and the APP/PS1/Tau triple transgenic mouse model by the herbal formula B401. In in vitro experiments, we showed that the herbal formula B401 treatment effectively reduces glutamate-induced excitotoxicity and acetylcholinesterase activity in Tet-On Aβ 42-GFP SH-SY5Y cells. In in vivo experiments, we found that oral B401 treatment effectively ameliorates neurocognitive dysfunctions of 3× Tg-AD mice via motor and cognitive behavior tests. By using magnetic resonance imaging, moorFLPI instruments, and chemiluminescence methods, we reported that oral B401 treatment effectively alleviates brain atrophy, improves subcutaneous blood flow, and reduces blood ROS in 3× Tg-AD mice. As observed from results of immunohistochemistry staining and western blotting, we found that oral B401 treatment significantly enhances expressions of neuroprotective proteins, while reducing expressions of AD derived proteins such as amyloid beta, phosphorylated Tau, neurofibrillary tangles, and 3-nitrotyrosine in the brain of 3× Tg-AD mice. Thus, the herbal formula B401 may have the potential to be developed into optimum TCM for AD patients.

Original languageEnglish
Article number1939052
JournalEvidence-based Complementary and Alternative Medicine
Volume2016
DOIs
Publication statusPublished - 2016 Jan 1

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Neuroprotective Agents
Alzheimer Disease
Neurofibrillary Tangles
Brain
Therapeutics
Acetylcholinesterase
Luminescence
Amyloid
Transgenic Mice
Atrophy
Glutamic Acid
Proteins
Western Blotting
Immunohistochemistry
Magnetic Resonance Imaging
B401 herbal formula
Staining and Labeling

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Neuroprotective Effects of the Herbal Formula B401 in Both Cell and Mouse Models of Alzheimer's Disease. / Hsu, Chih Hsiang; Wang, Sheue Er; Lin, Ching Lung; Hsiao, Chun Jen; Sheu, Shuenn Jyi; Wu, Chung Hsin.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2016, 1939052, 01.01.2016.

Research output: Contribution to journalArticle

Hsu, Chih Hsiang ; Wang, Sheue Er ; Lin, Ching Lung ; Hsiao, Chun Jen ; Sheu, Shuenn Jyi ; Wu, Chung Hsin. / Neuroprotective Effects of the Herbal Formula B401 in Both Cell and Mouse Models of Alzheimer's Disease. In: Evidence-based Complementary and Alternative Medicine. 2016 ; Vol. 2016.
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