Neonatal hypoxic-ischemic injury increases forebrain subventricular zone neurogenesis in the mouse

Jennifer M. Plane, Ruowen Liu, Tsu Wei Wang, Faye S. Silverstein, Jack M. Parent

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Neurogenesis persists throughout life in the rodent subventricular zone (SVZ)-olfactory bulb pathway and increases in the adult after brain insults. The influence of neonatal injury on SVZ neural precursors is unknown. We examined the effects of hypoxia-ischemia (HI) on neonatal mouse SVZ cell proliferation and neurogenesis. Postnatal day 10 (P10) mice underwent right carotid artery ligation followed by 10% O 2 exposure for 45 min. The SVZ area and hemispheric injury were quantified morphometrically 1-3 weeks later. Bromodeoxyuridine (BrdU) was used to label proliferating cells, and cell phenotypes of the progeny were identified by immunohistochemistry. HI significantly enlarged the ipsilateral SVZ at P18, P24, and P31, and increases in the SVZ area correlated directly with the degree of hemispheric damage. HI also stimulated cell proliferation and neurogenesis in the SVZ and peri-infarct striatum. Some newborn cells expressed a neuronal phenotype at P24, but not at P31, indicating that neurogenesis was short-lived. These results suggest that augmenting SVZ neuroblast recruitment and survival may improve neural repair after neonatal brain injury.

Original languageEnglish
Pages (from-to)585-595
Number of pages11
JournalNeurobiology of Disease
Volume16
Issue number3
DOIs
Publication statusPublished - 2004 Aug 1

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Lateral Ventricles
Neurogenesis
Prosencephalon
Wounds and Injuries
Ischemia
Cell Proliferation
Phenotype
Olfactory Pathways
Olfactory Bulb
Bromodeoxyuridine
Carotid Arteries
Brain Injuries
Ligation
Rodentia
Immunohistochemistry
Brain

Keywords

  • Bromodeoxyuridine
  • Doublecortin
  • Hypoxia-ischemia
  • Neural stem cell
  • Neuroblasts
  • Neurogenesis
  • Subventricular zone

ASJC Scopus subject areas

  • Neurology

Cite this

Neonatal hypoxic-ischemic injury increases forebrain subventricular zone neurogenesis in the mouse. / Plane, Jennifer M.; Liu, Ruowen; Wang, Tsu Wei; Silverstein, Faye S.; Parent, Jack M.

In: Neurobiology of Disease, Vol. 16, No. 3, 01.08.2004, p. 585-595.

Research output: Contribution to journalArticle

Plane, Jennifer M. ; Liu, Ruowen ; Wang, Tsu Wei ; Silverstein, Faye S. ; Parent, Jack M. / Neonatal hypoxic-ischemic injury increases forebrain subventricular zone neurogenesis in the mouse. In: Neurobiology of Disease. 2004 ; Vol. 16, No. 3. pp. 585-595.
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