Abstract
Yu-Ching Wu, Li-Yih Lin and Tsung-Han Lee (2003) Na+,K +,2CI--cotransporter: a novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus. Zoological Studies 42(1): 186-192. The abundance and location of the branchial Na+,K+2CI --cotransporter (NKCC) were examined in freshwater-and seawater-adapted tilapia. Immunoblots revealed 4 bands with molecular masses centered at 282, 208, 122, and 105 kDa, respectively. Gill NKCC in tilapia is upregulated after seawater adaptation. Confocal laser scanning micrographs showed that in seawater-adapted tilapia, NKCC as well as Na,K-ATPase exhibited identical diffuse distribution confined to the basal portion of branchial mitochondria-rich cells. However, in freshwater-adapted tilapia, NKCC was displayed only in the apical region of Na,K-ATPase-immunoreactive cells. Polarized distribution of NKCC makes it a novel marker for recognizing freshwater-or seawater-type MR cells in euryhaline tilapia. http://www.sinica. edu.tw/zool/zoolstud/42.1/186.pdf.
| Original language | English |
|---|---|
| Pages (from-to) | 186-192 |
| Number of pages | 7 |
| Journal | Zoological Studies |
| Volume | 42 |
| Issue number | 1 |
| Publication status | Published - 2003 Jan |
| Externally published | Yes |
Keywords
- Branchial epithelium
- Chloride cells
- NKCC
- Teleosts
ASJC Scopus subject areas
- Animal Science and Zoology