TY - JOUR
T1 - Na+,K+,2CI--cotransporter
T2 - A novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus
AU - Wu, Yu Ching
AU - Lin, Li Yih
AU - Lee, Tsung Han
PY - 2003/1
Y1 - 2003/1
N2 - Yu-Ching Wu, Li-Yih Lin and Tsung-Han Lee (2003) Na+,K +,2CI--cotransporter: a novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus. Zoological Studies 42(1): 186-192. The abundance and location of the branchial Na+,K+2CI --cotransporter (NKCC) were examined in freshwater-and seawater-adapted tilapia. Immunoblots revealed 4 bands with molecular masses centered at 282, 208, 122, and 105 kDa, respectively. Gill NKCC in tilapia is upregulated after seawater adaptation. Confocal laser scanning micrographs showed that in seawater-adapted tilapia, NKCC as well as Na,K-ATPase exhibited identical diffuse distribution confined to the basal portion of branchial mitochondria-rich cells. However, in freshwater-adapted tilapia, NKCC was displayed only in the apical region of Na,K-ATPase-immunoreactive cells. Polarized distribution of NKCC makes it a novel marker for recognizing freshwater-or seawater-type MR cells in euryhaline tilapia. http://www.sinica. edu.tw/zool/zoolstud/42.1/186.pdf.
AB - Yu-Ching Wu, Li-Yih Lin and Tsung-Han Lee (2003) Na+,K +,2CI--cotransporter: a novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus. Zoological Studies 42(1): 186-192. The abundance and location of the branchial Na+,K+2CI --cotransporter (NKCC) were examined in freshwater-and seawater-adapted tilapia. Immunoblots revealed 4 bands with molecular masses centered at 282, 208, 122, and 105 kDa, respectively. Gill NKCC in tilapia is upregulated after seawater adaptation. Confocal laser scanning micrographs showed that in seawater-adapted tilapia, NKCC as well as Na,K-ATPase exhibited identical diffuse distribution confined to the basal portion of branchial mitochondria-rich cells. However, in freshwater-adapted tilapia, NKCC was displayed only in the apical region of Na,K-ATPase-immunoreactive cells. Polarized distribution of NKCC makes it a novel marker for recognizing freshwater-or seawater-type MR cells in euryhaline tilapia. http://www.sinica. edu.tw/zool/zoolstud/42.1/186.pdf.
KW - Branchial epithelium
KW - Chloride cells
KW - NKCC
KW - Teleosts
UR - http://www.scopus.com/inward/record.url?scp=21144433321&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21144433321&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:21144433321
SN - 1021-5506
VL - 42
SP - 186
EP - 192
JO - Zoological Studies
JF - Zoological Studies
IS - 1
ER -