Na+,K+,2CI--cotransporter: A novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus

Yu Ching Wu, Li-Yih Lin, Tsung Han Lee

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Yu-Ching Wu, Li-Yih Lin and Tsung-Han Lee (2003) Na+,K +,2CI--cotransporter: a novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus. Zoological Studies 42(1): 186-192. The abundance and location of the branchial Na+,K+2CI --cotransporter (NKCC) were examined in freshwater-and seawater-adapted tilapia. Immunoblots revealed 4 bands with molecular masses centered at 282, 208, 122, and 105 kDa, respectively. Gill NKCC in tilapia is upregulated after seawater adaptation. Confocal laser scanning micrographs showed that in seawater-adapted tilapia, NKCC as well as Na,K-ATPase exhibited identical diffuse distribution confined to the basal portion of branchial mitochondria-rich cells. However, in freshwater-adapted tilapia, NKCC was displayed only in the apical region of Na,K-ATPase-immunoreactive cells. Polarized distribution of NKCC makes it a novel marker for recognizing freshwater-or seawater-type MR cells in euryhaline tilapia. http://www.sinica. edu.tw/zool/zoolstud/42.1/186.pdf.

Original languageEnglish
Pages (from-to)186-192
Number of pages7
JournalZoological Studies
Volume42
Issue number1
Publication statusPublished - 2003 Jan 1

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Tilapia (Cichlidae)
Oreochromis mossambicus
gills
mitochondria
seawater
sodium-potassium-exchanging ATPase
cells
lasers
molecular weight

Keywords

  • Branchial epithelium
  • Chloride cells
  • NKCC
  • Teleosts

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

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title = "Na+,K+,2CI--cotransporter: A novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus",
abstract = "Yu-Ching Wu, Li-Yih Lin and Tsung-Han Lee (2003) Na+,K +,2CI--cotransporter: a novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus. Zoological Studies 42(1): 186-192. The abundance and location of the branchial Na+,K+2CI --cotransporter (NKCC) were examined in freshwater-and seawater-adapted tilapia. Immunoblots revealed 4 bands with molecular masses centered at 282, 208, 122, and 105 kDa, respectively. Gill NKCC in tilapia is upregulated after seawater adaptation. Confocal laser scanning micrographs showed that in seawater-adapted tilapia, NKCC as well as Na,K-ATPase exhibited identical diffuse distribution confined to the basal portion of branchial mitochondria-rich cells. However, in freshwater-adapted tilapia, NKCC was displayed only in the apical region of Na,K-ATPase-immunoreactive cells. Polarized distribution of NKCC makes it a novel marker for recognizing freshwater-or seawater-type MR cells in euryhaline tilapia. http://www.sinica. edu.tw/zool/zoolstud/42.1/186.pdf.",
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