TY - JOUR
T1 - Nanomagnetic particles for SQUID-based magnetically labeled immunoassay
AU - Horng, H. E.
AU - Yang, S. Y.
AU - Huang, Y. W.
AU - Jiang, W. Q.
AU - Hong, C. Y.
AU - Yang, H. C.
N1 - Funding Information:
Manuscript received October 5, 2004. This work was supported by the NSC under Grants NSC93-2120-M-003-001, 93-2112-M-003-017, and 93-2112-M-002-041, and by MOE under Grant 91-N-FA01-2-4-2. H. E. Horng is with the Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan and also with the Department of Physics, National Taiwan Normal University, Taipei 116, Taiwan (e-mail: [email protected]). S. Y. Yang and Y. W. Huang are with the Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan. W. Q. Jiang is with the Department of Physics, National Taiwan University, Taipei 106, Taiwan and also with the Department of Chemistry, University of Science and Technology of China, Hefei, 230026, P. R. China. C.-Y. Hong is with the Department of Mechanical and Automation Engineering, Da-Yeh University, Chang-Hwa 515, Taiwan. H. C. Yang is with the Department of Physics, National Taiwan University, Taipei 106, Taiwan. Digital Object Identifier 10.1109/TASC.2005.849995
PY - 2005/6
Y1 - 2005/6
N2 - With the increasing importance of SQUID-based magnetically labeled immunoassay, the study on the synthesis of controllable sizes of magnetic nanoparticles plays a role to promote the accuracy of the immunoassay. In this work, Fe3O4 nano-particles coated with a suitable bio-probe (biotin) are synthesized through chemical co-precipitation process to probe the bio-target (avidin). Through the synthesis developed here, the particle hydrodynamic diameter can be adjusted from 30 to 90 nm, which provide candidates for probing various bio-targets in the future. The amount of the magnetically labeled avidin is then analyzed via measuring the saturated magnetization or the remanence of the sample by using a SQUID magnetometer.
AB - With the increasing importance of SQUID-based magnetically labeled immunoassay, the study on the synthesis of controllable sizes of magnetic nanoparticles plays a role to promote the accuracy of the immunoassay. In this work, Fe3O4 nano-particles coated with a suitable bio-probe (biotin) are synthesized through chemical co-precipitation process to probe the bio-target (avidin). Through the synthesis developed here, the particle hydrodynamic diameter can be adjusted from 30 to 90 nm, which provide candidates for probing various bio-targets in the future. The amount of the magnetically labeled avidin is then analyzed via measuring the saturated magnetization or the remanence of the sample by using a SQUID magnetometer.
KW - Immunoassay
KW - Nanomagnetic particles
KW - SQUIDs
UR - http://www.scopus.com/inward/record.url?scp=22044432025&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22044432025&partnerID=8YFLogxK
U2 - 10.1109/TASC.2005.849995
DO - 10.1109/TASC.2005.849995
M3 - Article
AN - SCOPUS:22044432025
SN - 1051-8223
VL - 15
SP - 668
EP - 671
JO - IEEE Transactions on Applied Superconductivity
JF - IEEE Transactions on Applied Superconductivity
IS - 2 PART I
ER -