Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics

Li Ling Yang, Chia Fen Hsieh, Yi Ren Chang, Jie Pan Shen, Yu Chung Chang, Chia Fu Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We combine pulsed laser, supercontinuum radiation source and fast single-photon counting peripherals to obtain a multifunctional micro/nano-scope. This provides us with better spatial and temporal resolution to observe fast dynamics. Performing fluorescence correlation spectroscopy for fast dynamics (<μs) with sub-diffraction limit resolution to observe the initial state of single-lipid dynamics in supported lipid bilayers and living cells is our goal. Lipid raft serves as a platform for recruiting signaling components of effective signal transduction. However, the dynamics of sub-200nm rapidly aggregated lipid rafts are still not elucidated in living cells. We here report our recent progress on the construction of this multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics study.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging V
DOIs
Publication statusPublished - 2012 Mar 5
EventSingle Molecule Spectroscopy and Superresolution Imaging V - San Francisco, CA, United States
Duration: 2012 Jan 212012 Jan 22

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8228
ISSN (Print)1605-7422

Other

OtherSingle Molecule Spectroscopy and Superresolution Imaging V
CountryUnited States
CitySan Francisco, CA
Period12/1/2112/1/22

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Yang, L. L., Hsieh, C. F., Chang, Y. R., Shen, J. P., Chang, Y. C., & Chou, C. F. (2012). Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics. In Single Molecule Spectroscopy and Superresolution Imaging V [822804] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8228). https://doi.org/10.1117/12.908179