Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics

Li Ling Yang, Chia Fen Hsieh, Yi Ren Chang, Jie Pan Shen, Yu Chung Chang, Chia Fu Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We combine pulsed laser, supercontinuum radiation source and fast single-photon counting peripherals to obtain a multifunctional micro/nano-scope. This provides us with better spatial and temporal resolution to observe fast dynamics. Performing fluorescence correlation spectroscopy for fast dynamics (<μs) with sub-diffraction limit resolution to observe the initial state of single-lipid dynamics in supported lipid bilayers and living cells is our goal. Lipid raft serves as a platform for recruiting signaling components of effective signal transduction. However, the dynamics of sub-200nm rapidly aggregated lipid rafts are still not elucidated in living cells. We here report our recent progress on the construction of this multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics study.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging V
DOIs
Publication statusPublished - 2012 Mar 5
EventSingle Molecule Spectroscopy and Superresolution Imaging V - San Francisco, CA, United States
Duration: 2012 Jan 212012 Jan 22

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8228
ISSN (Print)1605-7422

Other

OtherSingle Molecule Spectroscopy and Superresolution Imaging V
CountryUnited States
CitySan Francisco, CA
Period12/1/2112/1/22

Fingerprint

Lipids
lipids
Fluorescence
Scanning
fluorescence
scanning
rafts
Cells
Fluorescence Spectrometry
Lipid Bilayers
Signal transduction
Photons
Lipid bilayers
Signal Transduction
Lasers
radiation sources
Pulsed lasers
temporal resolution
Light sources
Radiation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Yang, L. L., Hsieh, C. F., Chang, Y. R., Shen, J. P., Chang, Y. C., & Chou, C. F. (2012). Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics. In Single Molecule Spectroscopy and Superresolution Imaging V [822804] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8228). https://doi.org/10.1117/12.908179

Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics. / Yang, Li Ling; Hsieh, Chia Fen; Chang, Yi Ren; Shen, Jie Pan; Chang, Yu Chung; Chou, Chia Fu.

Single Molecule Spectroscopy and Superresolution Imaging V. 2012. 822804 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8228).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yang, LL, Hsieh, CF, Chang, YR, Shen, JP, Chang, YC & Chou, CF 2012, Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics. in Single Molecule Spectroscopy and Superresolution Imaging V., 822804, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8228, Single Molecule Spectroscopy and Superresolution Imaging V, San Francisco, CA, United States, 12/1/21. https://doi.org/10.1117/12.908179
Yang LL, Hsieh CF, Chang YR, Shen JP, Chang YC, Chou CF. Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics. In Single Molecule Spectroscopy and Superresolution Imaging V. 2012. 822804. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.908179
Yang, Li Ling ; Hsieh, Chia Fen ; Chang, Yi Ren ; Shen, Jie Pan ; Chang, Yu Chung ; Chou, Chia Fu. / Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics. Single Molecule Spectroscopy and Superresolution Imaging V. 2012. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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