Morusin induces apoptosis and suppresses NF-κB activity in human colorectal cancer HT-29 cells

Jenq Chang Lee, Shen Jeu Won, Chien Lin Chao, Feng Ling Wu, Hsiao Sheng Liu, Pin Ling, Chun Nan Lin, Chun-Li Su

Research output: Contribution to journalArticle

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Abstract

Morusin is a pure compound isolated from root bark of Morus australis (Moraceae). In this study, we demonstrated that morusin significantly inhibited the growth and clonogenicity of human colorectal cancer HT-29 cells. Apoptosis induced by morusin was characterized by accumulation of cells at the sub-G1 phase, fragmentation of DNA, and condensation of chromatin. Morusin also inhibited the phosphorylation of IKK-α, IKK-β and IκB-α, increased expression of IκB-α, and suppressed nuclear translocation of NF-κB and its DNA binding activity. Dephosphorylation of NF-κB upstream regulators PI3K, Akt and PDK1 was also displayed. In addition, activation of caspase-8, change of mitochondrial membrane potential, release of cytochrome c and Smac/DIABLO, and activation of caspase-9 and -3 were observed at the early time point. Downregulation in the expression of Ku70 and XIAP was exhibited afterward. Caspase-8 or wide-ranging caspase inhibitor suppressed morusin-induced apoptosis. Therefore, the antitumor mechanism of morusin in HT-29 cells may be via activation of caspases and inhibition of NF-κB.

Original languageEnglish
Pages (from-to)236-242
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume372
Issue number1
DOIs
Publication statusPublished - 2008 Jul 18

Fingerprint

HT29 Cells
Human Activities
Colorectal Neoplasms
Apoptosis
Caspase 8
Chemical activation
Moraceae
Morus
Phosphorylation
Caspase Inhibitors
Caspase 9
Mitochondrial Membrane Potential
DNA
G1 Phase
DNA Fragmentation
Caspases
Cytochromes c
Phosphatidylinositol 3-Kinases
Caspase 3
Chromatin

Keywords

  • Apoptosis
  • Caspases
  • Colorectal cancer
  • Morusin
  • NF-κB

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Morusin induces apoptosis and suppresses NF-κB activity in human colorectal cancer HT-29 cells. / Lee, Jenq Chang; Won, Shen Jeu; Chao, Chien Lin; Wu, Feng Ling; Liu, Hsiao Sheng; Ling, Pin; Lin, Chun Nan; Su, Chun-Li.

In: Biochemical and Biophysical Research Communications, Vol. 372, No. 1, 18.07.2008, p. 236-242.

Research output: Contribution to journalArticle

Lee, Jenq Chang ; Won, Shen Jeu ; Chao, Chien Lin ; Wu, Feng Ling ; Liu, Hsiao Sheng ; Ling, Pin ; Lin, Chun Nan ; Su, Chun-Li. / Morusin induces apoptosis and suppresses NF-κB activity in human colorectal cancer HT-29 cells. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 372, No. 1. pp. 236-242.
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