Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye

Che Hang Yu; Shih Peng Tai; Chun Ta Kung; Wen Jeng Lee; Yi Fan Chan; Hsiang Lin Liu; Jiun Yi Lyu; Chi Kuang Sun

doi:10.1364/OL.33.000387

Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye

Che Hang Yu^*, Shih Peng Tai, Chun Ta Kung, Wen Jeng Lee, Yi Fan Chan, Hsiang Lin Liu, Jiun Yi Lyu, Chi Kuang Sun

^*Corresponding author for this work

Department of Physics

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

This paper reports a facile and effortless method to realize three-dimensional (3D) molecular third-harmonic-generation (THG) microscopy through the technique of resonance enhancement with absorbing dye. Hematoxylin, a popular absorbing stain, is applied as an example to verify the multiphoton resonant enhancement based on the 1230 nm excitation light and can selectively enhance THG yield at cell nuclear sites in the studied specimens, serving as a cell nucleus contrast agent. It is concluded that combining THG microscopy with the mature staining technique can readily achieve 3D molecular imaging without using fluorescence.

Original language	English
Pages (from-to)	387-389
Number of pages	3
Journal	Optics Letters
Volume	33
Issue number	4
DOIs	https://doi.org/10.1364/OL.33.000387
Publication status	Published - 2008 Feb 15

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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title = "Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye",

abstract = "This paper reports a facile and effortless method to realize three-dimensional (3D) molecular third-harmonic-generation (THG) microscopy through the technique of resonance enhancement with absorbing dye. Hematoxylin, a popular absorbing stain, is applied as an example to verify the multiphoton resonant enhancement based on the 1230 nm excitation light and can selectively enhance THG yield at cell nuclear sites in the studied specimens, serving as a cell nucleus contrast agent. It is concluded that combining THG microscopy with the mature staining technique can readily achieve 3D molecular imaging without using fluorescence.",

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AU - Yu, Che Hang

AU - Tai, Shih Peng

AU - Kung, Chun Ta

AU - Lee, Wen Jeng

AU - Chan, Yi Fan

AU - Liu, Hsiang Lin

AU - Lyu, Jiun Yi

AU - Sun, Chi Kuang

PY - 2008/2/15

Y1 - 2008/2/15

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AB - This paper reports a facile and effortless method to realize three-dimensional (3D) molecular third-harmonic-generation (THG) microscopy through the technique of resonance enhancement with absorbing dye. Hematoxylin, a popular absorbing stain, is applied as an example to verify the multiphoton resonant enhancement based on the 1230 nm excitation light and can selectively enhance THG yield at cell nuclear sites in the studied specimens, serving as a cell nucleus contrast agent. It is concluded that combining THG microscopy with the mature staining technique can readily achieve 3D molecular imaging without using fluorescence.

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Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye

Abstract

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