Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye

Che Hang Yu, Shih Peng Tai, Chun Ta Kung, Wen Jeng Lee, Yi Fan Chan, Hsiang Lin Liu, Jiun Yi Lyu, Chi Kuang Sun

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34 Citations (Scopus)

Abstract

This paper reports a facile and effortless method to realize three-dimensional (3D) molecular third-harmonic-generation (THG) microscopy through the technique of resonance enhancement with absorbing dye. Hematoxylin, a popular absorbing stain, is applied as an example to verify the multiphoton resonant enhancement based on the 1230 nm excitation light and can selectively enhance THG yield at cell nuclear sites in the studied specimens, serving as a cell nucleus contrast agent. It is concluded that combining THG microscopy with the mature staining technique can readily achieve 3D molecular imaging without using fluorescence.

Original languageEnglish
Pages (from-to)387-389
Number of pages3
JournalOptics Letters
Volume33
Issue number4
DOIs
Publication statusPublished - 2008 Feb 15

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Yu, C. H., Tai, S. P., Kung, C. T., Lee, W. J., Chan, Y. F., Liu, H. L., Lyu, J. Y., & Sun, C. K. (2008). Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye. Optics Letters, 33(4), 387-389. https://doi.org/10.1364/OL.33.000387