Modular organization of SARS coronavirus nucleocapsid protein

Chung Ke Chang, Shih Che Sue, Tsan Hung Yu, Chiu Min Hsieh, Cheng Kun Tsai, Yen Chieh Chiang, Shin Jye Lee, Hsin Hao Hsiao, Wen Jin Wu, Wei Lun Chang, Chun Hung Lin, Tai Huang Huang

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The SARS-CoV nucleocapsid (N) protein is a major antigen in severe acute respiratory syndrome. It binds to the viral RNA genome and forms the ribonucleoprotein core. The SARS-CoV N protein has also been suggested to be involved in other important functions in the viral life cycle. Here we show that the N protein consists of two non-interacting structural domains, the N-terminal RNA-binding domain (RBD) (residues 45-181) and the C-terminal dimerization domain (residues 248-365) (DD), surrounded by flexible linkers. The C-terminal domain exists exclusively as a dimer in solution. The flexible linkers are intrinsically disordered and represent potential interaction sites with other protein and protein-RNA partners. Bioinformatics reveal that other coronavirus N proteins could share the same modular organization. This study provides information on the domain structure partition of SARS-CoV N protein and insights into the differing roles of structured and disordered regions in coronavirus nucleocapsid proteins.

Original languageEnglish
Pages (from-to)59-72
Number of pages14
JournalJournal of Biomedical Science
Volume13
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

Fingerprint

SARS Virus
Proteins
RNA
Nucleocapsid
Ribonucleoproteins
Dimerization
Viral RNA
Nucleocapsid Proteins
Bioinformatics
Severe Acute Respiratory Syndrome
Dimers
Viral Genome
Life cycle
Life Cycle Stages
Computational Biology
Genes
Antigens
Coronavirus nucleocapsid protein

Keywords

  • Capsid protein
  • Coronavirus
  • Domain arrangement
  • Intrinsically disordered protein
  • NMR
  • Oligomerization
  • SARS

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Chang, C. K., Sue, S. C., Yu, T. H., Hsieh, C. M., Tsai, C. K., Chiang, Y. C., ... Huang, T. H. (2006). Modular organization of SARS coronavirus nucleocapsid protein. Journal of Biomedical Science, 13(1), 59-72. https://doi.org/10.1007/s11373-005-9035-9

Modular organization of SARS coronavirus nucleocapsid protein. / Chang, Chung Ke; Sue, Shih Che; Yu, Tsan Hung; Hsieh, Chiu Min; Tsai, Cheng Kun; Chiang, Yen Chieh; Lee, Shin Jye; Hsiao, Hsin Hao; Wu, Wen Jin; Chang, Wei Lun; Lin, Chun Hung; Huang, Tai Huang.

In: Journal of Biomedical Science, Vol. 13, No. 1, 01.01.2006, p. 59-72.

Research output: Contribution to journalArticle

Chang, CK, Sue, SC, Yu, TH, Hsieh, CM, Tsai, CK, Chiang, YC, Lee, SJ, Hsiao, HH, Wu, WJ, Chang, WL, Lin, CH & Huang, TH 2006, 'Modular organization of SARS coronavirus nucleocapsid protein', Journal of Biomedical Science, vol. 13, no. 1, pp. 59-72. https://doi.org/10.1007/s11373-005-9035-9
Chang CK, Sue SC, Yu TH, Hsieh CM, Tsai CK, Chiang YC et al. Modular organization of SARS coronavirus nucleocapsid protein. Journal of Biomedical Science. 2006 Jan 1;13(1):59-72. https://doi.org/10.1007/s11373-005-9035-9
Chang, Chung Ke ; Sue, Shih Che ; Yu, Tsan Hung ; Hsieh, Chiu Min ; Tsai, Cheng Kun ; Chiang, Yen Chieh ; Lee, Shin Jye ; Hsiao, Hsin Hao ; Wu, Wen Jin ; Chang, Wei Lun ; Lin, Chun Hung ; Huang, Tai Huang. / Modular organization of SARS coronavirus nucleocapsid protein. In: Journal of Biomedical Science. 2006 ; Vol. 13, No. 1. pp. 59-72.
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