Modification of morphology and function of integument mitochondria-rich cells in tilapia larvae (Oreochromis mossambicus) acclimated to ambient chloride levels

L. Y. Lin, P. P. Hwang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Similar to those of the gills of adults, three types of mitochondria-rich (MR) cells with different morphologies of apical surfaces (wavy convex, shallow basin, and deep hole) were identified on the integument of freshwater-acclimated tilapia larvae (Oreochromis mossambicus). The object of this study is to test the hypothesis that these subtype cells may represent MR cells equipped with variable efficiencies in Cl- uptake. Larvae acclimated to low-Cl- ([Cl-] = 0.001-0.007 mM) water developed higher densities of MR cells than those acclimated to high-C1- ([C1-] = 7.3-7.9 mM) water. The percentage of wavy-convex-type cells in total MR cells was higher in low-Cl--acclimated larvae than in high-Cl--acclimated larvae, which displayed only deep-hole type. In addition, Cl- influx rates of whole larva measured with 36Cl- showed a coincident correlation with MR cell densities, that is, low-Cl- larvae displayed higher Cl- influx rates than did high-Cl- larva, suggesting that tilapia larvae develop a higher density of MR cells with larger apical surfaces (wavy-convex type) to boost Cl- uptake in Cl--deficient water. The distinct types of apical surfaces may represent different phases of MR cells that possess different efficiencies of Cl- uptake. Increased apical membrane surface areas of MR cells may provide larvae with rapid regulation of Cl- before new MR cells differentiate.

Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalPhysiological and Biochemical Zoology
Volume74
Issue number4
DOIs
Publication statusPublished - 2001 Aug 14

Fingerprint

Tilapia (Cichlidae)
Tilapia
Oreochromis mossambicus
Mitochondria
integument
Larva
Chlorides
chlorides
mitochondria
larvae
cells
Water
uptake mechanisms
water
Fresh Water
Cell Count
surface area
gills
Membranes

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Animal Science and Zoology

Cite this

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abstract = "Similar to those of the gills of adults, three types of mitochondria-rich (MR) cells with different morphologies of apical surfaces (wavy convex, shallow basin, and deep hole) were identified on the integument of freshwater-acclimated tilapia larvae (Oreochromis mossambicus). The object of this study is to test the hypothesis that these subtype cells may represent MR cells equipped with variable efficiencies in Cl- uptake. Larvae acclimated to low-Cl- ([Cl-] = 0.001-0.007 mM) water developed higher densities of MR cells than those acclimated to high-C1- ([C1-] = 7.3-7.9 mM) water. The percentage of wavy-convex-type cells in total MR cells was higher in low-Cl--acclimated larvae than in high-Cl--acclimated larvae, which displayed only deep-hole type. In addition, Cl- influx rates of whole larva measured with 36Cl- showed a coincident correlation with MR cell densities, that is, low-Cl- larvae displayed higher Cl- influx rates than did high-Cl- larva, suggesting that tilapia larvae develop a higher density of MR cells with larger apical surfaces (wavy-convex type) to boost Cl- uptake in Cl--deficient water. The distinct types of apical surfaces may represent different phases of MR cells that possess different efficiencies of Cl- uptake. Increased apical membrane surface areas of MR cells may provide larvae with rapid regulation of Cl- before new MR cells differentiate.",
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N2 - Similar to those of the gills of adults, three types of mitochondria-rich (MR) cells with different morphologies of apical surfaces (wavy convex, shallow basin, and deep hole) were identified on the integument of freshwater-acclimated tilapia larvae (Oreochromis mossambicus). The object of this study is to test the hypothesis that these subtype cells may represent MR cells equipped with variable efficiencies in Cl- uptake. Larvae acclimated to low-Cl- ([Cl-] = 0.001-0.007 mM) water developed higher densities of MR cells than those acclimated to high-C1- ([C1-] = 7.3-7.9 mM) water. The percentage of wavy-convex-type cells in total MR cells was higher in low-Cl--acclimated larvae than in high-Cl--acclimated larvae, which displayed only deep-hole type. In addition, Cl- influx rates of whole larva measured with 36Cl- showed a coincident correlation with MR cell densities, that is, low-Cl- larvae displayed higher Cl- influx rates than did high-Cl- larva, suggesting that tilapia larvae develop a higher density of MR cells with larger apical surfaces (wavy-convex type) to boost Cl- uptake in Cl--deficient water. The distinct types of apical surfaces may represent different phases of MR cells that possess different efficiencies of Cl- uptake. Increased apical membrane surface areas of MR cells may provide larvae with rapid regulation of Cl- before new MR cells differentiate.

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